Detailed information of TA system TA063658

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2209516..2209741
• Replicon: chromosome
• Accession: NZ_CP015842
• Organism: Escherichia coli O157:H7 strain FRIK2533

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: A0A1Q4PF16
• Locus tag: A8V31_RS11915
• Protein ID: WP_000813258.1
• Coordinates: 2209516..2209671 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2209683..2209741 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A8V31_RS11865	2204519..2204950	-	432	WP_001302123.1	tellurite resistance protein	-
A8V31_RS11880	2205401..2206114	-	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
A8V31_RS11885	2206250..2206447	-	198	WP_000917763.1	hypothetical protein	-
A8V31_RS11890	2206672..2207226	-	555	WP_000640035.1	DUF1133 family protein	-
A8V31_RS11895	2207289..2207594	-	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
A8V31_RS11900	2207607..2208656	-	1050	WP_001265229.1	DUF968 domain-containing protein	-
A8V31_RS11905	2208658..2208930	-	273	WP_000191872.1	hypothetical protein	-
A8V31_RS11910	2209052..2209396	-	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
A8V31_RS11915	2209516..2209671	-	156	WP_000813258.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	2209683..2209741	+	59	-	-	Antitoxin
A8V31_RS11920	2209962..2210519	-	558	WP_000104474.1	DUF551 domain-containing protein	-
A8V31_RS11925	2210521..2210739	-	219	WP_000683609.1	DUF4014 family protein	-
A8V31_RS11930	2210867..2211178	-	312	WP_001289673.1	hypothetical protein	-
A8V31_RS11935	2211171..2211398	-	228	WP_000699809.1	hypothetical protein	-
A8V31_RS11940	2211395..2211676	-	282	WP_000603384.1	DNA-binding protein	-
A8V31_RS11945	2211709..2212425	-	717	WP_000450627.1	DUF1627 domain-containing protein	-
A8V31_RS11950	2212459..2212920	-	462	WP_000139447.1	replication protein	-
A8V31_RS30875	2212913..2213956	-	1044	WP_001262402.1	hypothetical protein	-
A8V31_RS11960	2214025..2214450	-	426	WP_000693878.1	Rha family transcriptional regulator	-
A8V31_RS11965	2214434..2214676	-	243	WP_000747948.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2176335..2261662	85327

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5767.95 Da
• Isoelectric Point: 7.7942

>T63658 WP_000813258.1 NZ_CP015842:c2209671-2209516 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 156 bp

>T63658 NZ_CP015842:c2209671-2209516 [Escherichia coli O157:H7]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTGCGAATCCGAACCGGTCAGACGGAGGTCGCTGTCTTCGTAGACTACGAATCTGAGAAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT63658 NZ_CP015842:2209683-2209741 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A1Q4PF16

Antitoxin