Detailed information of TA system TA063655

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 1846603..1846828
• Replicon: chromosome
• Accession: NZ_CP015842
• Organism: Escherichia coli O157:H7 strain FRIK2533

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: A8V31_RS10085
• Protein ID: WP_000813254.1
• Coordinates: 1846673..1846828 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	1846603..1846661 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A8V31_RS10035	1841856..1842809	+	954	WP_000095667.1	helix-turn-helix domain-containing protein	-
A8V31_RS10040	1842816..1843556	+	741	WP_000790456.1	ATP-binding protein	-
A8V31_RS10045	1843586..1844356	+	771	WP_000450888.1	DUF1627 domain-containing protein	-
A8V31_RS10050	1844372..1844767	+	396	WP_001118161.1	DUF977 family protein	-
A8V31_RS10055	1844824..1845180	+	357	WP_001302146.1	hypothetical protein	-
A8V31_RS10060	1845229..1845441	+	213	WP_000063625.1	hypothetical protein	-
A8V31_RS10065	1845477..1845848	+	372	WP_000137941.1	hypothetical protein	-
A8V31_RS10070	1845845..1846207	+	363	WP_000610379.1	DUF551 domain-containing protein	-
A8V31_RS10075	1846323..1846427	+	105	WP_001278450.1	hypothetical protein	-
-	1846603..1846661	-	59	-	-	Antitoxin
A8V31_RS10085	1846673..1846828	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
A8V31_RS31280	1847125..1847292	+	168	WP_000998188.1	hypothetical protein	-
A8V31_RS10090	1847358..1847636	+	279	WP_001304183.1	hypothetical protein	-
A8V31_RS10095	1847638..1848687	+	1050	Protein_1823	DUF968 domain-containing protein	-
A8V31_RS10100	1848700..1849074	+	375	WP_000904137.1	RusA family crossover junction endodeoxyribonuclease	-
A8V31_RS10105	1849071..1849892	+	822	WP_000762904.1	antitermination protein	-
A8V31_RS10110	1850119..1850316	+	198	WP_000917735.1	hypothetical protein	-
A8V31_RS10115	1850467..1851525	+	1059	WP_000483497.1	site-specific DNA-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	paa / nleA/espI / espM1	1772890..1889738	116848

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T63655 WP_000813254.1 NZ_CP015842:1846673-1846828 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T63655 NZ_CP015842:1846673-1846828 [Escherichia coli O157:H7]
ATGAAGCAGCAAAAGGCGATGCTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT63655 NZ_CP015842:c1846661-1846603 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACGTGTGTAGAGCATAGATATGGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin