63652

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1487396..1488191 Replicon chromosome
Accession NZ_CP015842
Organism Escherichia coli O157:H7 strain FRIK2533

Toxin (Protein)


Gene name yeeV Uniprot ID B7UP43
Locus tag A8V31_RS07790 Protein ID WP_000854914.1
Coordinates 1487817..1488191 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A7U9LM11
Locus tag A8V31_RS07785 Protein ID WP_001280953.1
Coordinates 1487396..1487770 (+) Length 125 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A8V31_RS07745 1482751..1483656 + 906 WP_000203541.1 chemotaxis protein -
A8V31_RS07750 1483653..1484723 + 1071 WP_000102669.1 patatin-like phospholipase family protein -
A8V31_RS07765 1485063..1485881 + 819 WP_001234682.1 DUF945 domain-containing protein -
A8V31_RS07770 1485972..1486457 + 486 WP_000214398.1 antirestriction protein -
A8V31_RS07775 1486473..1486949 + 477 WP_001186738.1 RadC family protein -
A8V31_RS07780 1487012..1487233 + 222 WP_000692323.1 DUF987 domain-containing protein -
A8V31_RS07785 1487396..1487770 + 375 WP_001280953.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
A8V31_RS07790 1487817..1488191 + 375 WP_000854914.1 TA system toxin CbtA family protein Toxin
A8V31_RS07795 1488188..1488679 + 492 WP_000976857.1 hypothetical protein -
A8V31_RS07800 1488691..1488888 + 198 WP_000839282.1 DUF957 domain-containing protein -
A8V31_RS07805 1488973..1489815 + 843 WP_001280481.1 DUF4942 domain-containing protein -
A8V31_RS07815 1490286..1491224 + 939 WP_000351284.1 glyoxylate/hydroxypyruvate reductase GhrA -
A8V31_RS07820 1491279..1492016 + 738 WP_000283664.1 zinc-binding phosphatase -
A8V31_RS07825 1492040..1492594 + 555 WP_001001917.1 molecular chaperone YcdY -
A8V31_RS07830 1492696..1493187 + 492 WP_001301587.1 DUF1097 domain-containing protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - csgG / csgF / csgE / csgD / csgB / csgA / csgC 1486473..1498755 12282


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(1-112)

Antitoxin

(18-117)


Sequences


Toxin        


Download         Length: 125 a.a.        Molecular weight: 14117.17 Da        Isoelectric Point: 7.7761

>T63652 WP_000854914.1 NZ_CP015842:1487817-1488191 [Escherichia coli O157:H7]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T63652 NZ_CP015842:1487817-1488191 [Escherichia coli O157:H7]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCTTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 13734.56 Da        Isoelectric Point: 6.6249

>AT63652 WP_001280953.1 NZ_CP015842:1487396-1487770 [Escherichia coli O157:H7]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLNAKGLTCEADTLGSCGYVYLAVYPTPALATTS

Download         Length: 375 bp

>AT63652 NZ_CP015842:1487396-1487770 [Escherichia coli O157:H7]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGAATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACTCGCAACCACCTCATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A7U9LXR5


Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LM11

References