Detailed information of TA system TA063650

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 1279974..1280199
• Replicon: chromosome
• Accession: NZ_CP015842
• Organism: Escherichia coli O157:H7 strain FRIK2533

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: A8V31_RS06585
• Protein ID: WP_000813263.1
• Coordinates: 1280044..1280199 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	1279974..1280032 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A8V31_RS06560	1275249..1276340	+	1092	WP_001205823.1	hypothetical protein	-
A8V31_RS06565	1276347..1277093	+	747	WP_000788751.1	ATP-binding protein	-
A8V31_RS06570	1277115..1277885	+	771	WP_000450992.1	DUF1627 domain-containing protein	-
A8V31_RS06575	1277901..1278314	+	414	WP_001151233.1	DUF977 family protein	-
A8V31_RS06580	1278666..1279439	-	774	WP_000160654.1	alpha/beta hydrolase	-
-	1279974..1280032	-	59	-	-	Antitoxin
A8V31_RS06585	1280044..1280199	+	156	WP_000813263.1	type I toxin-antitoxin system toxin HokD	Toxin
A8V31_RS06590	1280367..1280645	+	279	WP_001341388.1	hypothetical protein	-
A8V31_RS06595	1280647..1281696	+	1050	WP_001265172.1	DUF968 domain-containing protein	-
A8V31_RS06600	1281709..1282080	+	372	WP_001217436.1	RusA family crossover junction endodeoxyribonuclease	-
A8V31_RS06605	1282070..1282441	+	372	WP_000090264.1	antitermination protein	-
A8V31_RS06610	1282593..1283411	+	819	WP_000265265.1	CPBP family intramembrane metalloprotease	-
A8V31_RS06615	1283698..1283894	+	197	Protein_1197	TrmB family transcriptional regulator	-
A8V31_RS30695	1284032..1284745	+	714	WP_000261909.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5722.86 Da
• Isoelectric Point: 6.1531

>T63650 WP_000813263.1 NZ_CP015842:1280044-1280199 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T63650 NZ_CP015842:1280044-1280199 [Escherichia coli O157:H7]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAGTCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT63650 NZ_CP015842:c1280032-1279974 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Antitoxin