Detailed information of TA system TA063645

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 285670..286364
• Replicon: chromosome
• Accession: NZ_CP015842
• Organism: Escherichia coli O157:H7 strain FRIK2533

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: A8V31_RS01385
• Protein ID: WP_001263495.1
• Coordinates: 285966..286364 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: A8V31_RS01380
• Protein ID: WP_000554757.1
• Coordinates: 285670..285963 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A8V31_RS01360	281381..281878	+	498	Protein_247	REP-associated tyrosine transposase RayT	-
A8V31_RS01365	282023..283735	-	1713	Protein_248	flagellar biosynthesis protein FlhA	-
A8V31_RS01370	283707..284492	+	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
A8V31_RS01375	284563..285618	+	1056	WP_001226186.1	DNA polymerase IV	-
A8V31_RS01380	285670..285963	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
A8V31_RS01385	285966..286364	+	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
A8V31_RS01390	286374..286826	+	453	WP_001059871.1	GNAT family N-acetyltransferase	-
A8V31_RS01395	287145..287348	+	204	Protein_254	RNA ligase RtcB family protein	-
A8V31_RS01400	287347..287868	+	522	Protein_255	peptide chain release factor H	-
A8V31_RS01405	287925..289382	-	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
A8V31_RS01410	289643..290101	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
-	290697..290777	+	81	NuclAT_11	-	-
-	290697..290777	+	81	NuclAT_11	-	-
-	290697..290777	+	81	NuclAT_11	-	-
-	290697..290777	+	81	NuclAT_11	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T63645 WP_001263495.1 NZ_CP015842:285966-286364 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T63645 NZ_CP015842:285966-286364 [Escherichia coli O157:H7]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT63645 WP_000554757.1 NZ_CP015842:285670-285963 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT63645 NZ_CP015842:285670-285963 [Escherichia coli O157:H7]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6