Detailed information of TA system TA063633

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 108693..108947
• Replicon: plasmid pMS6198B
• Accession: NZ_CP015836
• Organism: Escherichia coli strain MS6198

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: MS6198_RS27050
• Protein ID: WP_032215358.1
• Coordinates: 108693..108899 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	108886..108947 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MS6198_RS27015	104052..104467	-	416	Protein_123	IS1 family transposase	-
MS6198_RS27020	104716..105117	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
MS6198_RS31365	105050..105307	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
MS6198_RS27030	105400..106053	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
MS6198_RS31805	106151..106291	-	141	WP_001333237.1	hypothetical protein	-
MS6198_RS27035	106992..107849	-	858	WP_029702152.1	incFII family plasmid replication initiator RepA	-
MS6198_RS27040	107842..107916	-	75	WP_001365705.1	RepA leader peptide Tap	-
MS6198_RS27045	108152..108409	-	258	WP_000083833.1	replication regulatory protein RepA	-
MS6198_RS27050	108693..108899	-	207	WP_032215358.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	108886..108947	+	62	NuclAT_0	-	Antitoxin
-	108886..108947	+	62	NuclAT_0	-	Antitoxin
-	108886..108947	+	62	NuclAT_0	-	Antitoxin
-	108886..108947	+	62	NuclAT_0	-	Antitoxin
MS6198_RS31605	109086..109352	-	267	WP_087757657.1	hypothetical protein	-
MS6198_RS27060	109727..110296	-	570	WP_029702150.1	DUF2726 domain-containing protein	-
MS6198_RS27065	110448..111074	-	627	WP_029702149.1	hypothetical protein	-
MS6198_RS27070	111486..111695	-	210	WP_039023209.1	hypothetical protein	-
MS6198_RS27075	111826..112386	-	561	WP_001567328.1	fertility inhibition protein FinO	-
MS6198_RS27080	112441..113187	-	747	WP_052273601.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(B) / blaTEM-1B / blaCTX-M-15 / erm(B) / mph(A) / sul1 / qacE / aadA5 / dfrA17	-	1..136327	136327

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7778.27 Da
• Isoelectric Point: 8.8807

>T63633 WP_032215358.1 NZ_CP015836:c108899-108693 [Escherichia coli]
MKYLNTTDCSLFLAERSKFMTKYALIGVLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T63633 NZ_CP015836:c108899-108693 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCCTTGCAGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GGTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAACTGAATATTCACAGGG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT63633 NZ_CP015836:108886-108947 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin