Detailed information of TA system TA063621

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4490983..4491585
• Replicon: chromosome
• Accession: NZ_CP015834
• Organism: Escherichia coli strain MS6198

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: MS6198_RS22055
• Protein ID: WP_000897305.1
• Coordinates: 4490983..4491294 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MS6198_RS22060
• Protein ID: WP_000356397.1
• Coordinates: 4491295..4491585 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MS6198_RS22025	4486013..4486798	+	786	WP_000059679.1	DeoR/GlpR family DNA-binding transcription regulator	-
MS6198_RS22030	4486897..4487496	+	600	WP_001314324.1	glucose-1-phosphatase	-
MS6198_RS22035	4487490..4488362	+	873	WP_000920762.1	virulence factor BrkB family protein	-
MS6198_RS22040	4488359..4488796	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
MS6198_RS22045	4488841..4489782	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
MS6198_RS22050	4489846..4490754	-	909	WP_001318161.1	alpha/beta hydrolase	-
MS6198_RS22055	4490983..4491294	+	312	WP_000897305.1	hypothetical protein	Toxin
MS6198_RS22060	4491295..4491585	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
MS6198_RS22070	4492190..4492408	+	219	WP_001298592.1	ribbon-helix-helix domain-containing protein	-
MS6198_RS22075	4492623..4493552	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
MS6198_RS22080	4493549..4494184	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
MS6198_RS22085	4494181..4495083	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T63621 WP_000897305.1 NZ_CP015834:4490983-4491294 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T63621 NZ_CP015834:4490983-4491294 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT63621 WP_000356397.1 NZ_CP015834:4491295-4491585 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT63621 NZ_CP015834:4491295-4491585 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7