Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 282444..283138 Replicon chromosome
Accession NZ_CP015834
Organism Escherichia coli strain MS6198

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag MS6198_RS01320 Protein ID WP_001263491.1
Coordinates 282740..283138 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag MS6198_RS01315 Protein ID WP_000554755.1
Coordinates 282444..282737 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MS6198_RS01290 277480..277740 - 261 WP_000729708.1 type II toxin-antitoxin system antitoxin DinJ -
MS6198_RS01295 277926..278699 + 774 WP_022645219.1 C40 family peptidase -
MS6198_RS01300 278797..280509 - 1713 Protein_249 flagellar biosynthesis protein FlhA -
MS6198_RS01305 280481..281266 + 786 WP_072224360.1 putative lateral flagellar export/assembly protein LafU -
MS6198_RS01310 281337..282392 + 1056 WP_022645222.1 DNA polymerase IV -
MS6198_RS01315 282444..282737 + 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
MS6198_RS01320 282740..283138 + 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
MS6198_RS01325 283148..283600 + 453 WP_023909048.1 GNAT family N-acetyltransferase -
MS6198_RS01330 283791..284930 + 1140 WP_022645224.1 RNA ligase RtcB family protein -
MS6198_RS01335 284927..285541 + 615 WP_022645225.1 peptide chain release factor H -
MS6198_RS01340 285603..286112 + 510 WP_001361775.1 hydrolase -
MS6198_RS01345 286129..286410 - 282 WP_022645226.1 hypothetical protein -
MS6198_RS01350 286419..287876 - 1458 WP_022645227.1 cytosol nonspecific dipeptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T63600 WP_001263491.1 NZ_CP015834:282740-283138 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T63600 NZ_CP015834:282740-283138 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACAGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT63600 WP_000554755.1 NZ_CP015834:282444-282737 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT63600 NZ_CP015834:282444-282737 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAACTGGTTAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References