Detailed information of TA system TA063591

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4472301..4472995
• Replicon: chromosome
• Accession: NZ_CP015832
• Organism: Escherichia coli O157 strain 180-PT54

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: A8L39_RS23185
• Protein ID: WP_001263495.1
• Coordinates: 4472301..4472699 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: A8L39_RS23190
• Protein ID: WP_000554757.1
• Coordinates: 4472702..4472995 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	4467888..4467968	-	81	NuclAT_11	-	-
-	4467888..4467968	-	81	NuclAT_11	-	-
-	4467888..4467968	-	81	NuclAT_11	-	-
-	4467888..4467968	-	81	NuclAT_11	-	-
A8L39_RS23160	4468564..4469022	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
A8L39_RS23165	4469283..4470740	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
A8L39_RS23170	4470797..4471318	-	522	Protein_4409	peptide chain release factor H	-
A8L39_RS23175	4471317..4471520	-	204	Protein_4410	RNA ligase RtcB family protein	-
A8L39_RS23180	4471839..4472291	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
A8L39_RS23185	4472301..4472699	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
A8L39_RS23190	4472702..4472995	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
A8L39_RS23195	4473047..4474102	-	1056	WP_001226186.1	DNA polymerase IV	-
A8L39_RS23200	4474173..4474958	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
A8L39_RS23205	4474930..4476642	+	1713	Protein_4416	flagellar biosynthesis protein FlhA	-
A8L39_RS33455	4476787..4477284	-	498	Protein_4417	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T63591 WP_001263495.1 NZ_CP015832:c4472699-4472301 [Escherichia coli O157]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T63591 NZ_CP015832:c4472699-4472301 [Escherichia coli O157]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT63591 WP_000554757.1 NZ_CP015832:c4472995-4472702 [Escherichia coli O157]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT63591 NZ_CP015832:c4472995-4472702 [Escherichia coli O157]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6