Detailed information of TA system TA063584

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3592292..3592517
• Replicon: chromosome
• Accession: NZ_CP015832
• Organism: Escherichia coli O157 strain 180-PT54

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: A8L39_RS19050
• Protein ID: WP_000813263.1
• Coordinates: 3592292..3592447 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	3592459..3592517 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A8L39_RS19015	3587746..3588459	-	714	WP_000261909.1	helix-turn-helix domain-containing protein	-
A8L39_RS19020	3588597..3588793	-	197	Protein_3624	TrmB family transcriptional regulator	-
A8L39_RS19025	3589080..3589898	-	819	WP_000265265.1	CPBP family intramembrane metalloprotease	-
A8L39_RS19030	3590050..3590421	-	372	WP_000090264.1	antitermination protein	-
A8L39_RS19035	3590411..3590782	-	372	WP_001217436.1	RusA family crossover junction endodeoxyribonuclease	-
A8L39_RS19040	3590795..3591844	-	1050	WP_001265172.1	DUF968 domain-containing protein	-
A8L39_RS32675	3591846..3592124	-	279	WP_001341388.1	hypothetical protein	-
A8L39_RS19050	3592292..3592447	-	156	WP_000813263.1	type I toxin-antitoxin system toxin HokD	Toxin
-	3592459..3592517	+	59	-	-	Antitoxin
A8L39_RS19055	3593052..3593825	+	774	WP_000160654.1	alpha/beta hydrolase	-
A8L39_RS19060	3594177..3594590	-	414	WP_001151233.1	DUF977 family protein	-
A8L39_RS19065	3594606..3595376	-	771	WP_000450992.1	DUF1627 domain-containing protein	-
A8L39_RS19070	3595398..3596144	-	747	WP_000788751.1	ATP-binding protein	-
A8L39_RS19075	3596151..3597242	-	1092	WP_001205823.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5722.86 Da
• Isoelectric Point: 6.1531

>T63584 WP_000813263.1 NZ_CP015832:c3592447-3592292 [Escherichia coli O157]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T63584 NZ_CP015832:c3592447-3592292 [Escherichia coli O157]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAGTCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT63584 NZ_CP015832:3592459-3592517 [Escherichia coli O157]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Antitoxin