Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3372048..3372843 Replicon chromosome
Accession NZ_CP015832
Organism Escherichia coli O157 strain 180-PT54

Toxin (Protein)


Gene name yeeV Uniprot ID B7UP43
Locus tag A8L39_RS17830 Protein ID WP_000854914.1
Coordinates 3372048..3372422 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A0B0VS41
Locus tag A8L39_RS17835 Protein ID WP_001280954.1
Coordinates 3372469..3372843 (-) Length 125 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A8L39_RS17790 3367052..3367543 - 492 WP_001301587.1 DUF1097 domain-containing protein -
A8L39_RS17795 3367645..3368199 - 555 WP_001001917.1 molecular chaperone YcdY -
A8L39_RS17800 3368223..3368960 - 738 WP_000283664.1 zinc-binding phosphatase -
A8L39_RS17805 3369015..3369953 - 939 WP_000351284.1 glyoxylate/hydroxypyruvate reductase GhrA -
A8L39_RS17815 3370424..3371266 - 843 WP_001280481.1 DUF4942 domain-containing protein -
A8L39_RS17820 3371351..3371548 - 198 WP_000839282.1 DUF957 domain-containing protein -
A8L39_RS17825 3371560..3372051 - 492 WP_000976858.1 hypothetical protein -
A8L39_RS17830 3372048..3372422 - 375 WP_000854914.1 TA system toxin CbtA family protein Toxin
A8L39_RS17835 3372469..3372843 - 375 WP_001280954.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
A8L39_RS17845 3373006..3373227 - 222 WP_001220314.1 DUF987 domain-containing protein -
A8L39_RS17850 3373290..3373766 - 477 WP_001186738.1 RadC family protein -
A8L39_RS17855 3373782..3374267 - 486 WP_000214398.1 antirestriction protein -
A8L39_RS17860 3374358..3375176 - 819 WP_001234682.1 DUF945 domain-containing protein -
A8L39_RS17870 3375516..3376586 - 1071 WP_000102669.1 patatin-like phospholipase family protein -
A8L39_RS17875 3376583..3377488 - 906 WP_000203541.1 chemotaxis protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - csgB / csgD / csgE / csgF / csgG 3363434..3374267 10833


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(1-112)

Antitoxin

(18-117)


Sequences


Toxin        


Download         Length: 125 a.a.        Molecular weight: 14117.17 Da        Isoelectric Point: 7.7761

>T63583 WP_000854914.1 NZ_CP015832:c3372422-3372048 [Escherichia coli O157]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T63583 NZ_CP015832:c3372422-3372048 [Escherichia coli O157]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 13718.51 Da        Isoelectric Point: 6.6249

>AT63583 WP_001280954.1 NZ_CP015832:c3372843-3372469 [Escherichia coli O157]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLNAKGLTCEADTLGSCGYVYLAVYPTPAPATTS

Download         Length: 375 bp

>AT63583 NZ_CP015832:c3372843-3372469 [Escherichia coli O157]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGAATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A7U9LXR5


Antitoxin

Source ID Structure
AlphaFold DB A0A0B0VS41

References