Detailed information of TA system TA063581

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3058326..3058551
• Replicon: chromosome
• Accession: NZ_CP015832
• Organism: Escherichia coli O157 strain 180-PT54

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: A8L39_RS15970
• Protein ID: WP_000813254.1
• Coordinates: 3058326..3058481 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	3058493..3058551 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A8L39_RS15935	3053611..3054669	-	1059	WP_000483509.1	site-specific DNA-methyltransferase	-
A8L39_RS15940	3054820..3055017	-	198	WP_000917735.1	hypothetical protein	-
A8L39_RS15945	3055244..3056065	-	822	WP_000762902.1	antitermination protein	-
A8L39_RS15950	3056062..3056436	-	375	WP_000904171.1	RusA family crossover junction endodeoxyribonuclease	-
A8L39_RS15955	3056449..3057498	-	1050	WP_001265156.1	DUF968 domain-containing protein	-
A8L39_RS32500	3057500..3057778	-	279	WP_012779355.1	hypothetical protein	-
A8L39_RS15965	3057848..3058105	-	258	WP_001217394.1	hypothetical protein	-
A8L39_RS15970	3058326..3058481	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
-	3058493..3058551	+	59	-	-	Antitoxin
A8L39_RS32505	3058727..3058831	-	105	WP_001278450.1	hypothetical protein	-
A8L39_RS15975	3058947..3059309	-	363	WP_000610377.1	DUF551 domain-containing protein	-
A8L39_RS15980	3059306..3059677	-	372	WP_000137941.1	hypothetical protein	-
A8L39_RS15985	3059713..3059925	-	213	WP_000063625.1	hypothetical protein	-
A8L39_RS15990	3059974..3060330	-	357	WP_001302146.1	hypothetical protein	-
A8L39_RS15995	3060387..3060782	-	396	WP_001118161.1	DUF977 family protein	-
A8L39_RS16000	3060798..3061568	-	771	WP_000537576.1	DUF1627 domain-containing protein	-
A8L39_RS32510	3061603..3062145	-	543	WP_157837342.1	replication protein	-
A8L39_RS33775	3062057..3063097	-	1041	WP_000020570.1	DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	ospG	3023255..3074071	50816

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T63581 WP_000813254.1 NZ_CP015832:c3058481-3058326 [Escherichia coli O157]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T63581 NZ_CP015832:c3058481-3058326 [Escherichia coli O157]
ATGAAGCAGCAAAAGGCGATGCTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT63581 NZ_CP015832:3058493-3058551 [Escherichia coli O157]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACGTGTGTAGAGCATAGATATGGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin