Detailed information of TA system TA063555

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symER/SymE(toxin)
• Location: 5141099..5141511
• Replicon: chromosome
• Accession: NZ_CP015831
• Organism: Escherichia coli O157 strain 644-PT8

Toxin (Protein)

• Gene name: symE
• Uniprot ID: Q8FA88
• Locus tag: A8L40_RS26630
• Protein ID: WP_000132630.1
• Coordinates: 5141170..5141511 (+)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	symR
Locus tag	-
Coordinates	5141099..5141175 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A8L40_RS26620	5137726..5139195	+	1470	WP_001302468.1	type I restriction-modification system subunit M	-
A8L40_RS26625	5139195..5140949	+	1755	WP_000110094.1	restriction endonuclease subunit S	-
-	5141099..5141175	-	77	NuclAT_14	-	Antitoxin
-	5141099..5141175	-	77	NuclAT_14	-	Antitoxin
-	5141099..5141175	-	77	NuclAT_14	-	Antitoxin
-	5141099..5141175	-	77	NuclAT_14	-	Antitoxin
-	5141099..5141175	-	77	NuclAT_15	-	Antitoxin
-	5141099..5141175	-	77	NuclAT_15	-	Antitoxin
-	5141099..5141175	-	77	NuclAT_15	-	Antitoxin
-	5141099..5141175	-	77	NuclAT_15	-	Antitoxin
A8L40_RS26630	5141170..5141511	+	342	WP_000132630.1	endoribonuclease SymE	Toxin
A8L40_RS32470	5141558..5142721	-	1164	WP_001304006.1	DUF1524 domain-containing protein	-
A8L40_RS26640	5142769..5143650	-	882	WP_001304007.1	DUF262 domain-containing protein	-
A8L40_RS26645	5143747..5143911	-	165	WP_000394274.1	DUF1127 domain-containing protein	-
A8L40_RS26650	5144088..5145500	+	1413	WP_000199295.1	PLP-dependent aminotransferase family protein	-
A8L40_RS35335	5145743..5145943	-	201	WP_001310455.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002655 Escherichia coli O157:H7 str. EDL933 5456776..5475497 0.003 View NC_002695 Escherichia coli O157:H7 str. Sakai 5426911..5445631 0.003	inside	Genomic island	-	espX6	5133298..5154537	21239

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12294.13 Da
• Isoelectric Point: 8.4982

>T63555 WP_000132630.1 NZ_CP015831:5141170-5141511 [Escherichia coli O157]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFATGTVVDVKVMEGCIVLTAQPPAAAESE
LMQSLRQVCKLSARKQRQVQEFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T63555 NZ_CP015831:5141170-5141511 [Escherichia coli O157]
ATGACTGATACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCGTTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACGGTGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGCCGAGAGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAGGCAGGTGCAGGAGTTTATTGGGGTGATTGC
GGGTAAACAGAAAGTTGCCTGA

Antitoxin

Nucleotide

• Length: 77 bp

>AT63555 NZ_CP015831:c5141175-5141099 [Escherichia coli O157]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: A0A0E0Y5T0

Antitoxin