Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) symER/SymE(toxin)
Location 5141099..5141511 Replicon chromosome
Accession NZ_CP015831
Organism Escherichia coli O157 strain 644-PT8

Toxin (Protein)


Gene name symE Uniprot ID Q8FA88
Locus tag A8L40_RS26630 Protein ID WP_000132630.1
Coordinates 5141170..5141511 (+) Length 114 a.a.

Antitoxin (RNA)


Gene name symR
Locus tag -
Coordinates 5141099..5141175 (-)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A8L40_RS26620 5137726..5139195 + 1470 WP_001302468.1 type I restriction-modification system subunit M -
A8L40_RS26625 5139195..5140949 + 1755 WP_000110094.1 restriction endonuclease subunit S -
- 5141099..5141175 - 77 NuclAT_14 - Antitoxin
- 5141099..5141175 - 77 NuclAT_14 - Antitoxin
- 5141099..5141175 - 77 NuclAT_14 - Antitoxin
- 5141099..5141175 - 77 NuclAT_14 - Antitoxin
- 5141099..5141175 - 77 NuclAT_15 - Antitoxin
- 5141099..5141175 - 77 NuclAT_15 - Antitoxin
- 5141099..5141175 - 77 NuclAT_15 - Antitoxin
- 5141099..5141175 - 77 NuclAT_15 - Antitoxin
A8L40_RS26630 5141170..5141511 + 342 WP_000132630.1 endoribonuclease SymE Toxin
A8L40_RS32470 5141558..5142721 - 1164 WP_001304006.1 DUF1524 domain-containing protein -
A8L40_RS26640 5142769..5143650 - 882 WP_001304007.1 DUF262 domain-containing protein -
A8L40_RS26645 5143747..5143911 - 165 WP_000394274.1 DUF1127 domain-containing protein -
A8L40_RS26650 5144088..5145500 + 1413 WP_000199295.1 PLP-dependent aminotransferase family protein -
A8L40_RS35335 5145743..5145943 - 201 WP_001310455.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - espX6 5133298..5154537 21239


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SymE-like

(20-71)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12294.13 Da        Isoelectric Point: 8.4982

>T63555 WP_000132630.1 NZ_CP015831:5141170-5141511 [Escherichia coli O157]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFATGTVVDVKVMEGCIVLTAQPPAAAESE
LMQSLRQVCKLSARKQRQVQEFIGVIAGKQKVA

Download         Length: 342 bp

>T63555 NZ_CP015831:5141170-5141511 [Escherichia coli O157]
ATGACTGATACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCGTTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACGGTGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGCCGAGAGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAGGCAGGTGCAGGAGTTTATTGGGGTGATTGC
GGGTAAACAGAAAGTTGCCTGA

Antitoxin


Download         Length: 77 bp

>AT63555 NZ_CP015831:c5141175-5141099 [Escherichia coli O157]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y5T0

Antitoxin

Download structure file

References