Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4800101..4800640 Replicon chromosome
Accession NZ_CP015831
Organism Escherichia coli O157 strain 644-PT8

Toxin (Protein)


Gene name yafQ Uniprot ID -
Locus tag A8L40_RS25020 Protein ID WP_001303998.1
Coordinates 4800380..4800640 (+) Length 87 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID C3TP07
Locus tag A8L40_RS25015 Protein ID WP_000729705.1
Coordinates 4800101..4800361 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A8L40_RS24995 4795855..4796640 - 786 WP_000207556.1 putative lateral flagellar export/assembly protein LafU -
A8L40_RS25000 4796612..4798324 + 1713 Protein_4784 flagellar biosynthesis protein FlhA -
A8L40_RS34660 4798469..4798966 - 498 Protein_4785 REP-associated tyrosine transposase RayT -
A8L40_RS25010 4799142..4799915 - 774 WP_000543891.1 C40 family peptidase -
A8L40_RS25015 4800101..4800361 + 261 WP_000729705.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
A8L40_RS25020 4800380..4800640 + 261 WP_001303998.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
A8L40_RS25025 4800796..4801536 + 741 WP_001225679.1 murein L,D-transpeptidase -
A8L40_RS25030 4801507..4802274 - 768 WP_001301698.1 class II glutamine amidotransferase -
A8L40_RS25035 4802379..4802957 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(3-85)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 87 a.a.        Molecular weight: 10026.71 Da        Isoelectric Point: 10.3188

>T63553 WP_001303998.1 NZ_CP015831:4800380-4800640 [Escherichia coli O157]
MNSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGT
HAALFG

Download         Length: 261 bp

>T63553 NZ_CP015831:4800380-4800640 [Escherichia coli O157]
TTGAACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAATAAATTGAAATATCTTAT
GACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAGGTTCATGGAAAGGTTATC
GCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGATTTGAGAGAACTGGAACT
CACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9377.74 Da        Isoelectric Point: 5.0758

>AT63553 WP_000729705.1 NZ_CP015831:4800101-4800361 [Escherichia coli O157]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGV

Download         Length: 261 bp

>AT63553 NZ_CP015831:4800101-4800361 [Escherichia coli O157]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References