Detailed information of TA system TA063545

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3847957..3848182
• Replicon: chromosome
• Accession: NZ_CP015831
• Organism: Escherichia coli O157 strain 644-PT8

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: A8L40_RS20495
• Protein ID: WP_000813263.1
• Coordinates: 3847957..3848112 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	3848124..3848182 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A8L40_RS20460	3843411..3844124	-	714	WP_000261909.1	helix-turn-helix domain-containing protein	-
A8L40_RS20465	3844262..3844458	-	197	Protein_3924	TrmB family transcriptional regulator	-
A8L40_RS20470	3844745..3845563	-	819	WP_000265265.1	CPBP family intramembrane metalloprotease	-
A8L40_RS20475	3845715..3846086	-	372	WP_000090264.1	antitermination protein	-
A8L40_RS20480	3846076..3846447	-	372	WP_001217436.1	RusA family crossover junction endodeoxyribonuclease	-
A8L40_RS20485	3846460..3847509	-	1050	WP_001265172.1	DUF968 domain-containing protein	-
A8L40_RS33710	3847511..3847789	-	279	WP_001341388.1	hypothetical protein	-
A8L40_RS20495	3847957..3848112	-	156	WP_000813263.1	type I toxin-antitoxin system toxin HokD	Toxin
-	3848124..3848182	+	59	-	-	Antitoxin
A8L40_RS20500	3848717..3849490	+	774	WP_000160654.1	alpha/beta hydrolase	-
A8L40_RS20505	3849842..3850255	-	414	WP_001151233.1	DUF977 family protein	-
A8L40_RS20510	3850271..3851041	-	771	WP_000450992.1	DUF1627 domain-containing protein	-
A8L40_RS20515	3851063..3851809	-	747	WP_000788751.1	ATP-binding protein	-
A8L40_RS20520	3851816..3852907	-	1092	WP_001205823.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5722.86 Da
• Isoelectric Point: 6.1531

>T63545 WP_000813263.1 NZ_CP015831:c3848112-3847957 [Escherichia coli O157]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T63545 NZ_CP015831:c3848112-3847957 [Escherichia coli O157]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAGTCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT63545 NZ_CP015831:3848124-3848182 [Escherichia coli O157]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Antitoxin