Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3625173..3625968 Replicon chromosome
Accession NZ_CP015831
Organism Escherichia coli O157 strain 644-PT8

Toxin (Protein)


Gene name yeeV Uniprot ID B7UP43
Locus tag A8L40_RS19265 Protein ID WP_000854914.1
Coordinates 3625173..3625547 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A0B0VS41
Locus tag A8L40_RS19270 Protein ID WP_001280954.1
Coordinates 3625594..3625968 (-) Length 125 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A8L40_RS19225 3620177..3620668 - 492 WP_001301587.1 DUF1097 domain-containing protein -
A8L40_RS19230 3620770..3621324 - 555 WP_001001917.1 molecular chaperone YcdY -
A8L40_RS19235 3621348..3622085 - 738 WP_000283664.1 zinc-binding phosphatase -
A8L40_RS19240 3622140..3623078 - 939 WP_000351284.1 glyoxylate/hydroxypyruvate reductase GhrA -
A8L40_RS19250 3623549..3624391 - 843 WP_001280481.1 DUF4942 domain-containing protein -
A8L40_RS19255 3624476..3624673 - 198 WP_000839282.1 DUF957 domain-containing protein -
A8L40_RS19260 3624685..3625176 - 492 WP_000976858.1 hypothetical protein -
A8L40_RS19265 3625173..3625547 - 375 WP_000854914.1 TA system toxin CbtA family protein Toxin
A8L40_RS19270 3625594..3625968 - 375 WP_001280954.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
A8L40_RS19280 3626131..3626352 - 222 WP_001220314.1 DUF987 domain-containing protein -
A8L40_RS19285 3626415..3626891 - 477 WP_001186738.1 RadC family protein -
A8L40_RS19290 3626907..3627392 - 486 WP_000214398.1 antirestriction protein -
A8L40_RS19295 3627483..3628301 - 819 WP_001234682.1 DUF945 domain-containing protein -
A8L40_RS19305 3628641..3629711 - 1071 WP_000102669.1 patatin-like phospholipase family protein -
A8L40_RS19310 3629708..3630613 - 906 WP_000203541.1 chemotaxis protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - csgB / csgD / csgE / csgF / csgG 3616559..3627392 10833


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(1-112)

Antitoxin

(18-117)


Sequences


Toxin        


Download         Length: 125 a.a.        Molecular weight: 14117.17 Da        Isoelectric Point: 7.7761

>T63544 WP_000854914.1 NZ_CP015831:c3625547-3625173 [Escherichia coli O157]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T63544 NZ_CP015831:c3625547-3625173 [Escherichia coli O157]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 13718.51 Da        Isoelectric Point: 6.6249

>AT63544 WP_001280954.1 NZ_CP015831:c3625968-3625594 [Escherichia coli O157]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLNAKGLTCEADTLGSCGYVYLAVYPTPAPATTS

Download         Length: 375 bp

>AT63544 NZ_CP015831:c3625968-3625594 [Escherichia coli O157]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGAATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A7U9LXR5


Antitoxin

Source ID Structure
AlphaFold DB A0A0B0VS41

References