Detailed information of TA system TA063542

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3252243..3252468
• Replicon: chromosome
• Accession: NZ_CP015831
• Organism: Escherichia coli O157 strain 644-PT8

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: A8L40_RS17085
• Protein ID: WP_000813254.1
• Coordinates: 3252243..3252398 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	3252410..3252468 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A8L40_RS17050	3247528..3248586	-	1059	WP_000483509.1	site-specific DNA-methyltransferase	-
A8L40_RS17055	3248737..3248934	-	198	WP_000917735.1	hypothetical protein	-
A8L40_RS17060	3249161..3249982	-	822	WP_000762902.1	antitermination protein	-
A8L40_RS17065	3249979..3250353	-	375	WP_000904171.1	RusA family crossover junction endodeoxyribonuclease	-
A8L40_RS17070	3250366..3251415	-	1050	WP_001265156.1	DUF968 domain-containing protein	-
A8L40_RS33330	3251417..3251695	-	279	WP_012779355.1	hypothetical protein	-
A8L40_RS17080	3251765..3252022	-	258	WP_001217394.1	hypothetical protein	-
A8L40_RS17085	3252243..3252398	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
-	3252410..3252468	+	59	-	-	Antitoxin
A8L40_RS33335	3252644..3252748	-	105	WP_001278450.1	hypothetical protein	-
A8L40_RS17090	3252864..3253226	-	363	WP_000610377.1	DUF551 domain-containing protein	-
A8L40_RS17095	3253223..3253594	-	372	WP_000137941.1	hypothetical protein	-
A8L40_RS17100	3253630..3253842	-	213	WP_000063625.1	hypothetical protein	-
A8L40_RS17105	3253891..3254247	-	357	WP_001302146.1	hypothetical protein	-
A8L40_RS17110	3254304..3254699	-	396	WP_001118161.1	DUF977 family protein	-
A8L40_RS17115	3254715..3255485	-	771	WP_000537576.1	DUF1627 domain-containing protein	-
A8L40_RS33340	3255520..3256062	-	543	WP_157837342.1	replication protein	-
A8L40_RS35010	3255974..3257014	-	1041	WP_000020570.1	DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	ospG / espM1 / nleF / nleH2 / nleA/espI / paa	3217812..3322163	104351

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T63542 WP_000813254.1 NZ_CP015831:c3252398-3252243 [Escherichia coli O157]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T63542 NZ_CP015831:c3252398-3252243 [Escherichia coli O157]
ATGAAGCAGCAAAAGGCGATGCTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT63542 NZ_CP015831:3252410-3252468 [Escherichia coli O157]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACGTGTGTAGAGCATAGATATGGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin