Detailed information of TA system    

experimentalExperimentally validated    

Overview


TA module


Type I Classification (family/domain) ibs-sib/-
Location 2153644..2153779 Replicon chromosome
Accession NC_000913
Organism Escherichia coli str. K-12 substr. MG1655
T1TAdb ID TA07163

Toxin (Protein)


Gene name ibs Uniprot ID C1P608
Locus tag b4668 Protein ID YP_002791248.1
Coordinates 2153681..2153737 (-) Length 19 a.a.

Antitoxin (RNA)


Gene name sib
Locus tag b4437
Coordinates 2153637..2153803 (+)

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
b2070 2149039..2150985 - 1947 NP_416574.1 putative serine/threonine-protein kinase YegI -
b2071 2151185..2151646 + 462 NP_416575.1 DUF2314 domain-containing protein YegJ -
b2072 2151711..2152472 - 762 NP_416576.1 protein-serine/threonine phosphatase PphC -
b2073 2152469..2153128 - 660 NP_416577.1 IPR002035 domain-containing protein YegL -
b4667 2153349..2153408 - 60 YP_002791247.1 toxic peptide IbsA -
- 2153637..2153803 + 167 - - Antitoxin
b4668 2153681..2153737 - 57 YP_002791248.1 putative toxic peptide IbsB Toxin
b2074 2154016..2155263 + 1248 NP_416578.2 multidrug efflux pump membrane fusion protein MdtA -
b2075 2155263..2158385 + 3123 NP_416579.1 multidrug efflux pump RND permease subunit MdtB -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(1-18)


Sequences


Toxin        


Download         Length: 19 a.a.        Molecular weight: 2063.71 Da        Isoelectric Point: 8.7571

>T6354 YP_002791248.1 NC_000913:c2153737-2153681 [Escherichia coli str. K-12 substr. MG1655]
MMKLLIIVVLLVISFPAY

Download         Length: 57 bp

>T6354 NC_000913:c2153737-2153681 [Escherichia coli str. K-12 substr. MG1655]
ATGATGAAGCTACTCATCATCGTGGTGCTCTTAGTCATAAGCTTCCCCGCTTACTAA

Antitoxin


Download         Length: 167 bp

>AT6354 NC_000913:2153637-2153803 [Escherichia coli str. K-12 substr. MG1655]
TTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCAC
GATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCG
CTGAAAT

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB C1P608


Antitoxin

Download structure file

References


(1) Elizabeth M Fozo et al. (2008) Repression of small toxic protein synthesis by the Sib and OhsC small RNAs. Molecular Microbiology 70(5):1076-93. [PubMed:18710431] experimental

(2) Elizabeth M Fozo et al. (2010) Abundance of type I toxin-antitoxin systems in bacteria: searches for new candidates and discovery of novel families. Nucleic Acids Research 38(11):3743-59. [PubMed:20156992] experimental


experimental experimental literature