Detailed information of TA system TA063539

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2895370..2895595
• Replicon: chromosome
• Accession: NZ_CP015831
• Organism: Escherichia coli O157 strain 644-PT8

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: A0A1Q4PF16
• Locus tag: A8L40_RS15300
• Protein ID: WP_000813258.1
• Coordinates: 2895440..2895595 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2895370..2895428 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A8L40_RS15250	2890435..2890677	+	243	WP_000747948.1	hypothetical protein	-
A8L40_RS15255	2890661..2891086	+	426	WP_000693878.1	Rha family transcriptional regulator	-
A8L40_RS34975	2891155..2892198	+	1044	WP_001262402.1	hypothetical protein	-
A8L40_RS33260	2892191..2892652	+	462	WP_000139447.1	replication protein	-
A8L40_RS15270	2892686..2893402	+	717	WP_000450627.1	DUF1627 domain-containing protein	-
A8L40_RS15275	2893435..2893716	+	282	WP_000603384.1	DNA-binding protein	-
A8L40_RS15280	2893713..2893940	+	228	WP_000699809.1	hypothetical protein	-
A8L40_RS15285	2893933..2894244	+	312	WP_001289673.1	hypothetical protein	-
A8L40_RS15290	2894372..2894590	+	219	WP_000683609.1	DUF4014 family protein	-
A8L40_RS15295	2894592..2895149	+	558	WP_000104474.1	DUF551 domain-containing protein	-
-	2895370..2895428	-	59	-	-	Antitoxin
A8L40_RS15300	2895440..2895595	+	156	WP_000813258.1	type I toxin-antitoxin system Hok family toxin	Toxin
A8L40_RS15305	2895715..2896059	+	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
A8L40_RS15310	2896181..2896453	+	273	WP_000191872.1	hypothetical protein	-
A8L40_RS15315	2896455..2897504	+	1050	WP_001265229.1	DUF968 domain-containing protein	-
A8L40_RS15320	2897517..2897822	+	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
A8L40_RS15325	2897885..2898439	+	555	WP_000640035.1	DUF1133 family protein	-
A8L40_RS34345	2898664..2898861	+	198	WP_000917763.1	hypothetical protein	-
A8L40_RS15335	2898997..2899710	+	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
A8L40_RS15350	2900161..2900592	+	432	WP_001302123.1	tellurite resistance protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	paa / espX7/nleL / nleG7'	2832530..2939599	107069

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5767.95 Da
• Isoelectric Point: 7.7942

>T63539 WP_000813258.1 NZ_CP015831:2895440-2895595 [Escherichia coli O157]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 156 bp

>T63539 NZ_CP015831:2895440-2895595 [Escherichia coli O157]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTGCGAATCCGAACCGGTCAGACGGAGGTCGCTGTCTTCGTAGACTACGAATCTGAGAAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT63539 NZ_CP015831:c2895428-2895370 [Escherichia coli O157]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A1Q4PF16

Antitoxin