Detailed information of TA system TA063460

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1168627..1169277
• Replicon: chromosome
• Accession: NZ_CP015774
• Organism: Lelliottia amnigena strain ZB04

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: A8A57_RS05520
• Protein ID: WP_064325149.1
• Coordinates: 1168936..1169277 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A1A9F849
• Locus tag: A8A57_RS05515
• Protein ID: WP_064325148.1
• Coordinates: 1168627..1168926 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A8A57_RS05500	1163759..1164838	+	1080	WP_064325146.1	YncE family protein	-
A8A57_RS05505	1164891..1167077	+	2187	WP_064328076.1	TonB-dependent siderophore receptor	-
A8A57_RS05510	1167190..1168584	+	1395	WP_064325147.1	phenylalanine transporter	-
A8A57_RS05515	1168627..1168926	-	300	WP_064325148.1	helix-turn-helix transcriptional regulator	Antitoxin
A8A57_RS05520	1168936..1169277	-	342	WP_064325149.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A8A57_RS05525	1169483..1170721	+	1239	WP_064328077.1	MFS transporter	-
A8A57_RS05530	1170737..1171762	+	1026	WP_064325150.1	sugar phosphate isomerase/epimerase	-
A8A57_RS05535	1171755..1172897	+	1143	WP_064325151.1	Gfo/Idh/MocA family oxidoreductase	-
A8A57_RS05540	1172973..1173944	+	972	WP_064325152.1	LacI family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-107)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-87)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13070.16 Da
• Isoelectric Point: 7.2149

>T63460 WP_064325149.1 NZ_CP015774:c1169277-1168936 [Lelliottia amnigena]
MWNVETTERFEEWLFRQSLSLKEDMLAALHILAEFGPQLGRPYVDTVKSSVYPNMKELRIQHAGDPIRAFFAFDPCRKAI
VLCAGDKTGCVEKQFYKEMIRLADAEFSKHLAK

Nucleotide

• Length: 342 bp

>T63460 NZ_CP015774:c1169277-1168936 [Lelliottia amnigena]
ATGTGGAATGTTGAAACTACTGAGAGATTTGAAGAATGGCTCTTTCGCCAGTCACTTTCGTTAAAGGAGGATATGTTGGC
TGCATTGCATATCCTTGCAGAATTCGGCCCGCAGCTCGGGCGCCCGTATGTGGATACCGTTAAATCTTCTGTGTATCCCA
ACATGAAAGAGCTCCGCATCCAGCATGCTGGCGATCCGATTCGGGCATTCTTCGCTTTCGATCCCTGTCGCAAAGCCATA
GTGCTCTGCGCTGGTGATAAAACTGGTTGCGTTGAAAAACAATTCTACAAAGAAATGATTAGGTTGGCTGATGCCGAATT
CAGTAAACACCTGGCGAAGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10911.70 Da
• Isoelectric Point: 9.8629

>AT63460 WP_064325148.1 NZ_CP015774:c1168926-1168627 [Lelliottia amnigena]
MATLKELMAQQSVESQARIEAKVAEMRRIVALHQLREELNVSQTELATAMGVKQPTLAKIEQPGNDPRLSTLKRYVAALG
GELSLDVTLPNGKRIAFQI

Nucleotide

• Length: 300 bp

>AT63460 NZ_CP015774:c1168926-1168627 [Lelliottia amnigena]
ATGGCAACGTTAAAAGAACTCATGGCACAACAAAGCGTGGAAAGTCAGGCACGCATTGAAGCTAAGGTTGCAGAAATGCG
CCGTATAGTGGCACTTCACCAACTGCGTGAGGAACTCAATGTTTCGCAGACGGAGCTCGCCACGGCAATGGGCGTAAAGC
AGCCTACGCTGGCTAAAATCGAACAACCGGGTAATGACCCACGCTTGTCCACGCTAAAACGCTATGTCGCAGCGCTCGGT
GGAGAGTTGAGTCTTGATGTCACATTGCCTAATGGGAAACGGATTGCGTTTCAAATTTGA

Structures

Antitoxin

• AlphaFold DB: A0A1A9F849