Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 77027..77757 Replicon chromosome
Accession NZ_CP015774
Organism Lelliottia amnigena strain ZB04

Toxin (Protein)


Gene name higB Uniprot ID A0A1A9F6V2
Locus tag A8A57_RS00385 Protein ID WP_064324240.1
Coordinates 77027..77341 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A1A9F679
Locus tag A8A57_RS00390 Protein ID WP_064324241.1
Coordinates 77338..77757 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A8A57_RS00355 72690..73778 + 1089 WP_064324237.1 cellulase family glycosylhydrolase -
A8A57_RS00360 73681..74865 - 1185 WP_064328044.1 multidrug efflux MFS transporter EmrD -
A8A57_RS00365 75041..75874 - 834 WP_064324238.1 DMT family transporter -
A8A57_RS00370 75937..76383 - 447 WP_064324239.1 GNAT family N-acetyltransferase -
A8A57_RS00375 76448..76537 - 90 WP_071599731.1 type I toxin-antitoxin system toxin TisB -
A8A57_RS00385 77027..77341 + 315 WP_064324240.1 type II toxin-antitoxin system HigB family toxin Toxin
A8A57_RS00390 77338..77757 + 420 WP_064324241.1 helix-turn-helix domain-containing protein Antitoxin
A8A57_RS00395 77907..78005 + 99 WP_071892292.1 ilvB operon leader peptide IvbL -
A8A57_RS00400 78113..79801 + 1689 WP_064324242.1 acetolactate synthase large subunit -
A8A57_RS00405 79805..80092 + 288 WP_064324243.1 acetolactate synthase small subunit -
A8A57_RS00410 80166..80759 + 594 WP_064324244.1 transcriptional regulator UhpA -
A8A57_RS00415 80756..82255 + 1500 WP_064324245.1 signal transduction histidine-protein kinase/phosphatase UhpB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(21-93)

Antitoxin

Antitoxin HigA

(1-139)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12481.65 Da        Isoelectric Point: 10.2747

>T63457 WP_064324240.1 NZ_CP015774:77027-77341 [Lelliottia amnigena]
MHLISMKALLDAVRQFPQYREELLFLGKIIEKSHCPTPAALKKLFPTLDNFKYLDKHYVIDIANNNLRVVALIFFESQKF
YVRHVFNHKEYDRFTEKHRTKGKK

Download         Length: 315 bp

>T63457 NZ_CP015774:77027-77341 [Lelliottia amnigena]
ATGCATTTAATCTCAATGAAAGCTCTTTTGGATGCGGTCAGGCAGTTTCCTCAATACAGGGAAGAGCTTCTTTTTCTGGG
CAAAATTATCGAAAAAAGCCATTGCCCGACGCCTGCTGCGCTTAAAAAACTCTTTCCAACGCTGGATAATTTTAAGTATC
TCGATAAGCATTATGTGATTGATATTGCAAACAACAATCTCAGAGTTGTGGCTCTCATTTTCTTTGAAAGCCAAAAGTTT
TATGTGCGCCATGTTTTCAATCATAAGGAATATGACCGTTTTACGGAGAAACATCGCACTAAGGGGAAGAAATGA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15265.72 Da        Isoelectric Point: 5.1643

>AT63457 WP_064324241.1 NZ_CP015774:77338-77757 [Lelliottia amnigena]
MTIVAEAIKATHALVAAVPLLGEHPSEKDYNDALELVEYLLVNEPGSPLLDIVCARISHYEARQPDMVALRQEMNAIPAG
IAVLKTLMDQYNLTLSDFPDEIGSKSMVSRVLNGQRQLTLNHIKKLAARFGVSPALFIE

Download         Length: 420 bp

>AT63457 NZ_CP015774:77338-77757 [Lelliottia amnigena]
ATGACGATCGTCGCTGAAGCAATTAAAGCTACGCATGCCCTTGTTGCCGCTGTTCCTCTGTTAGGTGAACATCCGAGCGA
GAAGGATTACAACGATGCTCTGGAACTGGTTGAGTATTTGCTCGTCAATGAACCCGGTAGTCCCTTACTGGATATTGTCT
GTGCAAGAATCAGCCATTATGAGGCGCGTCAACCTGACATGGTTGCCTTACGCCAGGAGATGAACGCCATACCTGCCGGG
ATTGCAGTGTTAAAAACACTGATGGATCAGTACAACCTGACCCTATCTGATTTTCCGGACGAAATTGGCAGTAAATCAAT
GGTTTCAAGGGTGCTTAATGGCCAACGTCAGCTCACGTTAAACCATATTAAAAAGCTGGCTGCACGCTTTGGCGTATCGC
CCGCGCTCTTTATCGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1A9F6V2

Antitoxin

Source ID Structure
AlphaFold DB A0A1A9F679

References