Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Gp49-HTH_37
Location 2197748..2198616 Replicon chromosome
Accession NZ_CP015773
Organism Mycobacterium tuberculosis variant bovis strain SP38

Toxin (Protein)


Gene name higB Uniprot ID P9WJA4
Locus tag SZ58_RS10575 Protein ID WP_010886136.1
Coordinates 2197748..2198125 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0TLM0
Locus tag SZ58_RS10580 Protein ID WP_003409886.1
Coordinates 2198167..2198616 (+) Length 150 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SZ58_RS10525 2193537..2193989 - 453 WP_010950636.1 lipoprotein -
SZ58_RS10530 2194053..2194454 + 402 WP_003409869.1 hypothetical protein -
SZ58_RS10535 2194447..2194629 - 183 WP_003409870.1 hypothetical protein -
SZ58_RS10540 2194743..2195093 - 351 WP_003409871.1 hypothetical protein -
SZ58_RS10545 2195104..2196006 - 903 WP_003409874.1 hypothetical protein -
SZ58_RS10550 2196027..2196218 - 192 WP_003409876.1 hypothetical protein -
SZ58_RS10555 2196219..2196515 - 297 WP_003409877.1 hypothetical protein -
SZ58_RS10560 2196755..2196970 + 216 WP_003409878.1 antitoxin -
SZ58_RS10565 2196967..2197278 + 312 WP_003409881.1 type II toxin-antitoxin system VapC family toxin -
SZ58_RS21960 2197252..2197773 - 522 WP_010950637.1 hypothetical protein -
SZ58_RS10575 2197748..2198125 + 378 WP_010886136.1 type II toxin-antitoxin system toxin HigB Toxin
SZ58_RS10580 2198167..2198616 + 450 WP_003409886.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
SZ58_RS10585 2198613..2199158 + 546 WP_003409891.1 SecB-like chaperone -
SZ58_RS10590 2199047..2199661 - 615 WP_003901296.1 hypothetical protein -
SZ58_RS10595 2199710..2200006 - 297 WP_003409896.1 type II toxin-antitoxin system RelE/ParE family toxin -
SZ58_RS10600 2200003..2200254 - 252 WP_003409899.1 type II toxin-antitoxin system ParD family antitoxin -
SZ58_RS10605 2200241..2200735 + 495 WP_003899099.1 hypothetical protein -
SZ58_RS10610 2200895..2201302 - 408 WP_003409913.1 type II toxin-antitoxin system VapC family toxin -
SZ58_RS10615 2201306..2201578 - 273 WP_003899100.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
SZ58_RS10620 2201611..2202831 - 1221 WP_003409919.1 TetR family transcriptional regulator Mce3R -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(27-113)

Antitoxin

Cro/C1-type helix-turn-helix domain

(44-91)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14397.77 Da        Isoelectric Point: 10.8862

>T63429 WP_010886136.1 NZ_CP015773:2197748-2198125 [Mycobacterium tuberculosis variant bovis]
VPPPDPAAMGTWKFFRASVDGRPVFKKEFDKLPDQARAALIVLMQRYLVGDLAAGSIKPIRGDILELRWHEANNHFRVLF
FRWGQHPVALTAFYKNQQKTPKTKIETALDRQKIWKRAFGDTPPI

Download         Length: 378 bp

>T63429 NZ_CP015773:2197748-2198125 [Mycobacterium tuberculosis variant bovis]
GTGCCGCCCCCTGATCCAGCCGCCATGGGTACCTGGAAGTTCTTCCGGGCATCTGTGGATGGCCGGCCGGTATTCAAGAA
GGAGTTCGACAAGCTTCCTGATCAGGCCCGGGCCGCGCTGATCGTGCTAATGCAGCGGTATCTCGTCGGCGACCTCGCCG
CAGGGAGCATCAAACCGATTCGTGGCGACATTCTGGAGTTGCGATGGCATGAGGCGAACAACCACTTCCGGGTACTGTTC
TTCCGCTGGGGCCAGCATCCCGTAGCGCTGACAGCGTTCTACAAGAACCAGCAGAAGACTCCCAAGACGAAGATCGAGAC
GGCCCTGGACCGGCAGAAAATCTGGAAAAGAGCCTTCGGCGACACCCCACCGATCTGA

Antitoxin


Download         Length: 150 a.a.        Molecular weight: 16760.16 Da        Isoelectric Point: 8.0771

>AT63429 WP_003409886.1 NZ_CP015773:2198167-2198616 [Mycobacterium tuberculosis variant bovis]
MSIDFPLGDDLAGYIAEAIAADPSFKGTLEDAEEARRLVDALIALRKHCQLSQVEVAKRMGVRQPTVSGFEKEPSDPKLS
TLQRYARALDARLRLVLEVPTLREVPTWHRLSSYRGSARDHQVRVGADKEILMQTNWARHISVRQVEVA

Download         Length: 450 bp

>AT63429 NZ_CP015773:2198167-2198616 [Mycobacterium tuberculosis variant bovis]
ATGAGCATTGACTTCCCTTTGGGTGACGACCTCGCCGGCTATATTGCCGAGGCGATTGCGGCTGATCCCAGCTTCAAAGG
CACTCTCGAAGACGCCGAGGAGGCACGCAGGCTGGTCGATGCGCTGATTGCGCTGCGCAAGCACTGCCAGCTGAGCCAGG
TTGAGGTTGCTAAGCGTATGGGGGTGCGCCAGCCCACCGTGAGCGGTTTCGAGAAGGAACCCAGCGACCCCAAACTGTCT
ACGCTGCAACGTTATGCCCGTGCATTGGACGCCCGGCTGCGGCTGGTGCTCGAAGTTCCCACGCTTCGCGAAGTGCCTAC
GTGGCATCGGCTCTCCTCTTATCGGGGCTCCGCACGGGACCACCAGGTCCGGGTGGGTGCAGACAAGGAAATCCTGATGC
AGACGAACTGGGCCCGCCACATTTCGGTTCGGCAGGTTGAGGTGGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7AWK
PDB 7NBU

Antitoxin

Source ID Structure
PDB 5MTW
AlphaFold DB G0TLM0

References