Detailed information of TA system TA063348

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2217687..2218411
• Replicon: chromosome
• Accession: NZ_CP015750
• Organism: Pectobacterium wasabiae CFBP 3304

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A1D7Z112
• Locus tag: A7983_RS10045
• Protein ID: WP_005973726.1
• Coordinates: 2217687..2217998 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: A7983_RS10050
• Protein ID: WP_005973724.1
• Coordinates: 2217995..2218411 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7983_RS10020	2213290..2214024	+	735	WP_005973736.1	type I-E CRISPR-associated protein Cas5/CasD	-
A7983_RS10025	2214024..2214671	+	648	WP_005973734.1	type I-E CRISPR-associated protein Cas6/Cse3/CasE	-
A7983_RS10030	2214699..2215616	+	918	WP_005973732.1	type I-E CRISPR-associated endonuclease Cas1	-
A7983_RS10035	2215617..2215910	+	294	WP_005973730.1	type I-E CRISPR-associated endoribonuclease Cas2	-
A7983_RS10040	2216711..2217082	-	372	WP_005973728.1	helix-turn-helix domain-containing protein	-
A7983_RS10045	2217687..2217998	+	312	WP_005973726.1	type II toxin-antitoxin system HigB family toxin	Toxin
A7983_RS10050	2217995..2218411	+	417	WP_005973724.1	helix-turn-helix domain-containing protein	Antitoxin
A7983_RS10055	2219396..2219854	+	459	WP_005973722.1	hypothetical protein	-
A7983_RS10060	2219936..2221288	-	1353	WP_005973720.1	aspartate aminotransferase family protein	-
A7983_RS10065	2221322..2222590	-	1269	WP_039479226.1	MFS transporter	-
A7983_RS10070	2222745..2223191	+	447	WP_005973716.1	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (83-136)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12319.20 Da
• Isoelectric Point: 9.8535

>T63348 WP_005973726.1 NZ_CP015750:2217687-2217998 [Pectobacterium wasabiae CFBP 3304]
MHVISRAPFDTAIAQFPNQAAALADLYRVIKREMYATPDDMKKRFPSLDRMKYREKWWVIDIGGGHLRVMFFADFERGKI
FIKHITSHAEYDSLTEYYRRNKE

Nucleotide

• Length: 312 bp

>T63348 NZ_CP015750:2217687-2217998 [Pectobacterium wasabiae CFBP 3304]
ATGCATGTTATTTCGCGGGCACCTTTTGACACTGCGATCGCCCAGTTCCCGAATCAGGCGGCAGCACTGGCTGACTTATA
TCGGGTTATCAAACGCGAAATGTATGCAACGCCGGACGATATGAAAAAACGCTTCCCCAGCTTAGACAGGATGAAGTACA
GGGAAAAGTGGTGGGTTATTGATATTGGTGGTGGGCATCTTCGGGTGATGTTTTTCGCTGATTTTGAGCGAGGGAAAATT
TTCATTAAGCACATCACATCTCATGCTGAGTACGACAGTCTGACAGAGTATTACCGGAGGAATAAAGAATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15493.76 Da
• Isoelectric Point: 4.4920

>AT63348 WP_005973724.1 NZ_CP015750:2217995-2218411 [Pectobacterium wasabiae CFBP 3304]
MMYADAIKAANNLTSIVPFLGGSTSRKDYEDALKLTEYLIESEPDSPLIDMLTAKIDKYEEDAPEFAVFNERIKALPAGI
ALLRVLMDQHELTQSDFETEIGKKSLVSRILNGERTLTLDHMRALAKRFDIPVSAFID

Nucleotide

• Length: 417 bp

>AT63348 NZ_CP015750:2217995-2218411 [Pectobacterium wasabiae CFBP 3304]
ATGATGTATGCAGACGCCATCAAGGCCGCTAACAACCTGACGAGTATTGTACCGTTCCTCGGGGGGAGCACCTCTCGTAA
AGATTATGAGGATGCGCTGAAACTTACCGAATACTTGATCGAGAGCGAACCTGATAGCCCGCTAATCGATATGCTGACGG
CTAAAATTGACAAATATGAAGAGGATGCGCCGGAGTTCGCAGTCTTCAATGAACGTATTAAAGCGCTCCCTGCTGGCATC
GCTTTACTGCGTGTACTTATGGATCAGCATGAGCTAACGCAGAGTGATTTTGAAACTGAGATCGGTAAAAAGTCTCTGGT
ATCTCGCATTCTAAATGGTGAGCGTACACTAACGCTTGATCACATGAGAGCACTTGCCAAACGATTTGATATTCCCGTGA
GTGCTTTTATTGATTAG

Structures

Toxin

• AlphaFold DB: A0A1D7Z112