Detailed information of TA system TA063345

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1636193..1636865
• Replicon: chromosome
• Accession: NZ_CP015750
• Organism: Pectobacterium wasabiae CFBP 3304

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: A7983_RS07325
• Protein ID: WP_005976715.1
• Coordinates: 1636193..1636579 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A1D7YZI4
• Locus tag: A7983_RS07330
• Protein ID: WP_005976713.1
• Coordinates: 1636566..1636865 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7983_RS07305	1632534..1633682	-	1149	WP_005976723.1	galactonate dehydratase	-
A7983_RS07310	1633679..1634296	-	618	WP_005976721.1	2-dehydro-3-deoxy-6-phosphogalactonate aldolase	-
A7983_RS07315	1634280..1635176	-	897	WP_005976719.1	2-dehydro-3-deoxygalactonokinase	-
A7983_RS07320	1635173..1635871	-	699	WP_005976717.1	D-galactonate utilization transcriptional regulator DgoR	-
A7983_RS07325	1636193..1636579	+	387	WP_005976715.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A7983_RS07330	1636566..1636865	+	300	WP_005976713.1	XRE family transcriptional regulator	Antitoxin
A7983_RS07335	1636903..1638702	-	1800	WP_039479027.1	ABC transporter ATP-binding protein	-
A7983_RS07340	1638742..1639650	-	909	WP_005976709.1	ABC transporter permease	-
A7983_RS07345	1639647..1640624	-	978	WP_005976707.1	ABC transporter permease	-
A7983_RS07350	1640624..1641631	-	1008	WP_005976705.1	nucleoside hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (19-113)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14847.88 Da
• Isoelectric Point: 4.8667

>T63345 WP_005976715.1 NZ_CP015750:1636193-1636579 [Pectobacterium wasabiae CFBP 3304]
MEVYRLAWKIVTRALFDTWFDEQTEAVQEEVLAHLAILEEDGPQLGRPQVDQIKGSNYKNMKELRIQVGGHPFRTFFAFD
RTRKAIILCAGDKKGEDEKLFYERMIKIADAEFTSHLISPEVKEDDNA

Nucleotide

• Length: 387 bp

>T63345 NZ_CP015750:1636193-1636579 [Pectobacterium wasabiae CFBP 3304]
ATGGAGGTTTACCGATTGGCATGGAAAATCGTCACACGGGCGCTATTCGATACGTGGTTTGATGAGCAAACCGAAGCAGT
ACAGGAAGAGGTTTTAGCACACCTTGCCATACTCGAAGAGGATGGCCCACAGCTAGGGCGACCACAGGTTGATCAGATTA
AAGGCTCAAACTACAAAAATATGAAGGAGCTTCGGATTCAGGTTGGCGGCCACCCATTCCGAACATTTTTCGCGTTTGAC
CGGACACGTAAAGCGATCATTTTATGTGCGGGAGATAAGAAAGGCGAAGATGAAAAATTGTTCTACGAACGTATGATTAA
GATCGCAGACGCTGAGTTTACATCGCATCTGATATCACCGGAGGTAAAAGAAGATGACAACGCTTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11094.17 Da
• Isoelectric Point: 10.6442

>AT63345 WP_005976713.1 NZ_CP015750:1636566-1636865 [Pectobacterium wasabiae CFBP 3304]
MTTLKEMMAKRSPESRARIEARTAEIRQEITLAKLREELNMSQMQLAAVLGVKQPSIVKMEKVENDPKLSTLKRYVTALG
GKLTLDVTLPDGKRIALTL

Nucleotide

• Length: 300 bp

>AT63345 NZ_CP015750:1636566-1636865 [Pectobacterium wasabiae CFBP 3304]
ATGACAACGCTTAAAGAAATGATGGCTAAACGTTCACCGGAGAGTCGCGCCCGCATTGAAGCGCGCACGGCTGAGATCCG
TCAGGAAATCACCTTAGCGAAACTACGCGAAGAGCTGAACATGTCGCAGATGCAGCTTGCAGCAGTACTGGGCGTCAAGC
AGCCCTCTATTGTGAAGATGGAGAAGGTTGAAAATGACCCAAAACTATCAACGCTGAAACGTTATGTCACTGCTCTAGGT
GGCAAGCTTACGCTAGATGTGACACTGCCTGATGGTAAGCGTATCGCACTGACTTTATAA

Structures

Antitoxin

• AlphaFold DB: A0A1D7YZI4