Detailed information of TA system TA063341

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3625587..3626305
• Replicon: chromosome
• Accession: NZ_CP015749
• Organism: Pectobacterium parmentieri strain RNS 08-42-1A

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A8B3FNM7
• Locus tag: A8F97_RS16410
• Protein ID: WP_033070820.1
• Coordinates: 3626000..3626305 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A8B3FM67
• Locus tag: A8F97_RS16405
• Protein ID: WP_033070769.1
• Coordinates: 3625587..3626003 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A8F97_RS16390	3621113..3622384	+	1272	WP_033070821.1	MFS transporter	-
A8F97_RS16395	3622415..3623767	+	1353	WP_033070770.1	aspartate aminotransferase family protein	-
A8F97_RS16400	3623848..3624306	-	459	WP_012822197.1	hypothetical protein	-
A8F97_RS16405	3625587..3626003	-	417	WP_033070769.1	helix-turn-helix domain-containing protein	Antitoxin
A8F97_RS16410	3626000..3626305	-	306	WP_033070820.1	type II toxin-antitoxin system HigB family toxin	Toxin
A8F97_RS16415	3626457..3626717	+	261	WP_012822193.1	type II toxin-antitoxin system RelE/ParE family toxin	-
A8F97_RS16420	3626791..3627162	+	372	WP_012822192.1	helix-turn-helix domain-containing protein	-
A8F97_RS16425	3628391..3628684	-	294	WP_005973730.1	type I-E CRISPR-associated endoribonuclease Cas2	-
A8F97_RS16430	3628685..3629602	-	918	WP_012822191.1	type I-E CRISPR-associated endonuclease Cas1	-
A8F97_RS16435	3629630..3630277	-	648	WP_012822190.1	type I-E CRISPR-associated protein Cas6/Cse3/CasE	-
A8F97_RS16440	3630277..3631011	-	735	WP_012822189.1	type I-E CRISPR-associated protein Cas5/CasD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (18-90)

Antitoxin

• Cro/C1-type helix-turn-helix domain (83-134)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 12010.78 Da
• Isoelectric Point: 10.0386

>T63341 WP_033070820.1 NZ_CP015749:c3626305-3626000 [Pectobacterium parmentieri]
VFSRAPFDTAIAQFPNQAAALADLYRTIKREIYATPDDMKKRFPSLDRMKYREKWWVIDIGGGHLRVMFFADFERGKIFI
KHITSHAEYGSLTEYYRRNKE

Nucleotide

• Length: 306 bp

>T63341 NZ_CP015749:c3626305-3626000 [Pectobacterium parmentieri]
GTGTTCTCCCGGGCACCTTTTGACACTGCAATCGCCCAATTCCCTAATCAGGCGGCAGCACTGGCTGACCTATACCGGAC
GATAAAGCGTGAAATATATGCAACGCCGGACGATATGAAAAAACGTTTCCCCAGCTTAGACAGGATGAAGTACAGGGAAA
AGTGGTGGGTTATTGATATTGGTGGTGGGCATCTTCGGGTGATGTTTTTCGCTGATTTTGAGCGAGGGAAAATTTTTATC
AAACACATCACATCCCATGCAGAGTACGGCAGTCTGACAGAATATTACCGGAGGAATAAAGAATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15233.53 Da
• Isoelectric Point: 4.3708

>AT63341 WP_033070769.1 NZ_CP015749:c3626003-3625587 [Pectobacterium parmentieri]
MMYADAIKAANNLTSIVPLLGGSTSCKDYEDALKLIEYLLESEPDSPLIDMLTAKIDKYEEDAPEFVAFNERIKALPAGI
ALLRILMDQHGLTQSDFEAEIGKKSLVSRILNGERTLTVDHMSALAKRFDIPVSAFVD

Nucleotide

• Length: 417 bp

>AT63341 NZ_CP015749:c3626003-3625587 [Pectobacterium parmentieri]
ATGATGTACGCAGACGCAATCAAGGCCGCTAACAATCTGACGAGTATCGTACCGCTCCTCGGAGGTAGCACCTCTTGTAA
AGATTATGAGGATGCGCTGAAACTCATTGAATATTTACTCGAGAGCGAGCCTGATAGCCCGCTAATCGATATGCTGACGG
CTAAAATTGACAAATATGAAGAGGATGCGCCAGAGTTTGTAGCTTTCAACGAGCGTATTAAAGCGCTCCCTGCTGGTATC
GCTTTGCTGCGCATACTTATGGATCAGCATGGGCTAACGCAAAGTGACTTTGAAGCTGAGATCGGTAAAAAATCTCTGGT
ATCCCGAATTCTAAATGGTGAGCGTACACTAACGGTTGATCACATGAGCGCACTTGCTAAACGATTTGATATTCCCGTGA
GTGCTTTTGTTGATTAG

Structures

Toxin

• AlphaFold DB: A0A8B3FNM7

Antitoxin

• AlphaFold DB: A0A8B3FM67