Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-VapI
Location 1408776..1409354 Replicon chromosome
Accession NZ_CP015736
Organism Shinella sp. HZN7

Toxin (Protein)


Gene name higB Uniprot ID A0A1A9FY39
Locus tag shn_RS06800 Protein ID WP_064329735.1
Coordinates 1408776..1409054 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A1A9FY06
Locus tag shn_RS06805 Protein ID WP_064329736.1
Coordinates 1409067..1409354 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
shn_RS06770 1404023..1404955 - 933 WP_064329729.1 N-acetyl-gamma-glutamyl-phosphate reductase -
shn_RS06775 1405089..1406042 - 954 WP_064329730.1 agmatinase -
shn_RS06780 1406173..1406649 - 477 WP_064329731.1 30S ribosomal protein S9 -
shn_RS06785 1406652..1407116 - 465 WP_064329732.1 50S ribosomal protein L13 -
shn_RS06790 1407346..1407777 - 432 WP_064329733.1 PaaI family thioesterase -
shn_RS06795 1407916..1408731 + 816 WP_064329734.1 enoyl-CoA hydratase -
shn_RS06800 1408776..1409054 + 279 WP_064329735.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
shn_RS06805 1409067..1409354 + 288 WP_064329736.1 HigA family addiction module antidote protein Antitoxin
shn_RS06810 1409358..1410194 - 837 WP_064329737.1 DMT family transporter -
shn_RS06815 1410435..1410872 + 438 WP_064329738.1 CoA-binding protein -
shn_RS06820 1410938..1411366 + 429 WP_064329739.1 CoA-binding protein -
shn_RS06825 1411485..1412768 + 1284 WP_064329740.1 O-acetylhomoserine aminocarboxypropyltransferase -
shn_RS06830 1412824..1413168 + 345 WP_064329741.1 cupin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(6-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(7-64)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10455.95 Da        Isoelectric Point: 9.8012

>T63327 WP_064329735.1 NZ_CP015736:1408776-1409054 [Shinella sp. HZN7]
MIKSIADTATRQFVETGKSRFSGLDVARAMARLNVLNAARSLDDISPLRSIRLHKLTGDRKGQWAMTINGPWRLCFRFEN
GDAYDVEIVDYH

Download         Length: 279 bp

>T63327 NZ_CP015736:1408776-1409054 [Shinella sp. HZN7]
ATGATCAAGTCCATTGCGGACACGGCGACACGGCAGTTTGTTGAGACGGGAAAGTCGCGGTTTTCCGGGCTCGACGTCGC
AAGGGCGATGGCGCGTTTGAATGTCCTCAATGCCGCGCGAAGCCTGGACGACATATCGCCCTTGCGCTCGATCAGGCTTC
ACAAATTGACCGGTGACCGCAAGGGGCAATGGGCAATGACGATCAACGGGCCTTGGCGCCTTTGTTTTCGTTTCGAAAAT
GGCGATGCCTACGATGTCGAGATCGTCGACTATCATTGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10355.79 Da        Isoelectric Point: 7.2040

>AT63327 WP_064329736.1 NZ_CP015736:1409067-1409354 [Shinella sp. HZN7]
MFEVHPGSILKEELEARQMSGNALALALRINSGRVSEILNGKRGISADTALRLGRYFGNDPQFWLGLQAAHDLAVAQRES
GDRILREVLPAQTAA

Download         Length: 288 bp

>AT63327 NZ_CP015736:1409067-1409354 [Shinella sp. HZN7]
ATGTTCGAGGTTCATCCAGGTTCGATCCTGAAGGAAGAACTCGAAGCGCGACAGATGAGCGGCAACGCGCTTGCGCTCGC
GCTGCGGATCAATTCCGGCCGTGTGTCGGAAATATTGAACGGCAAGCGCGGGATCTCCGCGGATACGGCGCTTCGCCTGG
GCCGCTATTTCGGTAACGACCCGCAGTTCTGGCTGGGCTTGCAGGCCGCGCATGATCTCGCGGTCGCCCAGCGTGAAAGC
GGTGACAGGATATTGCGCGAAGTCCTGCCGGCCCAGACGGCCGCATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1A9FY39

Antitoxin

Source ID Structure
AlphaFold DB A0A1A9FY06

References