Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/-
Location 5413271..5413926 Replicon chromosome
Accession NZ_CP015638
Organism Pseudomonas fluorescens strain L111

Toxin (Protein)


Gene name higB Uniprot ID A0A855K4I9
Locus tag A7319_RS24045 Protein ID WP_024077334.1
Coordinates 5413582..5413926 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A855K566
Locus tag A7319_RS24040 Protein ID WP_024077333.1
Coordinates 5413271..5413585 (-) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A7319_RS24025 5409511..5410524 + 1014 WP_069076982.1 GGDEF domain-containing protein -
A7319_RS24030 5410742..5411992 + 1251 WP_024077330.1 aspartate aminotransferase family protein -
A7319_RS30645 5411989..5412153 - 165 Protein_4809 hypothetical protein -
A7319_RS24035 5412495..5413211 + 717 WP_024077332.1 hypothetical protein -
A7319_RS24040 5413271..5413585 - 315 WP_024077333.1 helix-turn-helix domain-containing protein Antitoxin
A7319_RS24045 5413582..5413926 - 345 WP_024077334.1 toxin HigB-2 Toxin
A7319_RS24050 5414020..5415111 - 1092 WP_069076996.1 pyridoxal phosphate-dependent aminotransferase -
A7319_RS24055 5415259..5416146 - 888 WP_024077336.1 heme o synthase -
A7319_RS24060 5416158..5416493 - 336 WP_003176003.1 cytochrome o ubiquinol oxidase subunit IV -
A7319_RS24065 5416493..5417119 - 627 WP_024077337.1 cytochrome o ubiquinol oxidase subunit III -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(42-94)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 13327.19 Da        Isoelectric Point: 9.8860

>T63282 WP_024077334.1 NZ_CP015638:c5413926-5413582 [Pseudomonas fluorescens]
MRSIFFETSSFTATVGHYLTDDEYRLLQNEMQWNPLAGDVMPRTGGFRKLRWSDGKRGKGKRSGLRVIYYWLTTDGHFWM
FAIYNKDELENLTADQEKALKKAIEAELKKRGTP

Download         Length: 345 bp

>T63282 NZ_CP015638:c5413926-5413582 [Pseudomonas fluorescens]
ATGCGTTCTATTTTCTTCGAGACTTCATCGTTTACCGCCACCGTAGGTCATTACCTGACGGATGATGAATATCGATTGCT
GCAAAACGAGATGCAGTGGAACCCTTTGGCTGGCGATGTGATGCCCCGTACAGGAGGGTTTCGCAAGCTACGGTGGTCAG
ATGGCAAGCGTGGTAAAGGAAAGCGTAGTGGCTTACGGGTTATTTACTACTGGCTCACCACTGATGGTCACTTTTGGATG
TTTGCTATCTACAACAAAGACGAATTGGAAAATTTGACTGCCGATCAAGAGAAGGCACTTAAAAAGGCCATAGAGGCCGA
ACTGAAAAAACGAGGTACCCCATGA

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 11789.63 Da        Isoelectric Point: 10.3067

>AT63282 WP_024077333.1 NZ_CP015638:c5413585-5413271 [Pseudomonas fluorescens]
MKKRDLFAELMQGVSDMTAHREGKITLRQSTVEDKPVPEVSAQEIVALRDKLHMSQSVFARSIRTSPGTLRNWEQDKSKP
NAQAALLIRLVEKFPDMVDRLATV

Download         Length: 315 bp

>AT63282 NZ_CP015638:c5413585-5413271 [Pseudomonas fluorescens]
ATGAAAAAGCGCGATCTGTTTGCAGAGTTGATGCAGGGCGTTAGCGACATGACTGCTCACCGAGAAGGCAAAATCACTCT
TCGGCAAAGCACTGTGGAGGACAAGCCCGTTCCTGAGGTGTCAGCCCAAGAGATCGTGGCACTGCGGGATAAATTGCATA
TGTCACAGTCGGTGTTTGCCCGGAGCATACGGACGAGCCCCGGCACGCTGAGAAACTGGGAACAGGACAAATCCAAACCC
AATGCTCAGGCCGCACTCCTGATCAGGCTGGTTGAAAAGTTTCCGGATATGGTGGATCGATTGGCGACCGTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A855K4I9

Antitoxin

Source ID Structure
AlphaFold DB A0A855K566

References