63279

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/-
Location 5497562..5498217 Replicon chromosome
Accession NZ_CP015637
Organism Pseudomonas fluorescens strain L321

Toxin (Protein)


Gene name higB Uniprot ID A0A855K4I9
Locus tag A7317_RS24515 Protein ID WP_024077334.1
Coordinates 5497562..5497906 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A855K566
Locus tag A7317_RS24520 Protein ID WP_024077333.1
Coordinates 5497903..5498217 (+) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A7317_RS24495 5494369..5494995 + 627 WP_024077337.1 cytochrome o ubiquinol oxidase subunit III -
A7317_RS24500 5494995..5495330 + 336 WP_003176003.1 cytochrome o ubiquinol oxidase subunit IV -
A7317_RS24505 5495342..5496229 + 888 WP_024077336.1 heme o synthase -
A7317_RS24510 5496377..5497468 + 1092 WP_069076996.1 pyridoxal phosphate-dependent aminotransferase -
A7317_RS24515 5497562..5497906 + 345 WP_024077334.1 toxin HigB-2 Toxin
A7317_RS24520 5497903..5498217 + 315 WP_024077333.1 helix-turn-helix domain-containing protein Antitoxin
A7317_RS24525 5498277..5498993 - 717 WP_024077332.1 hypothetical protein -
A7317_RS24530 5499503..5499847 - 345 WP_003230905.1 cupin domain-containing protein -
A7317_RS24535 5500301..5500951 + 651 WP_069076997.1 arylformamidase -
A7317_RS24540 5500948..5501805 + 858 WP_069076998.1 tryptophan 2,3-dioxygenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(42-94)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 13327.19 Da        Isoelectric Point: 9.8860

>T63279 WP_024077334.1 NZ_CP015637:5497562-5497906 [Pseudomonas fluorescens]
MRSIFFETSSFTATVGHYLTDDEYRLLQNEMQWNPLAGDVMPRTGGFRKLRWSDGKRGKGKRSGLRVIYYWLTTDGHFWM
FAIYNKDELENLTADQEKALKKAIEAELKKRGTP

Download         Length: 345 bp

>T63279 NZ_CP015637:5497562-5497906 [Pseudomonas fluorescens]
ATGCGTTCTATTTTCTTCGAGACTTCATCGTTTACCGCCACCGTAGGTCATTACCTGACGGATGATGAATATCGATTGCT
GCAAAACGAGATGCAGTGGAACCCTTTGGCTGGCGATGTGATGCCCCGTACAGGAGGGTTTCGCAAGCTACGGTGGTCAG
ATGGCAAGCGTGGTAAAGGAAAGCGTAGTGGCTTACGGGTTATTTACTACTGGCTCACCACTGATGGTCACTTTTGGATG
TTTGCTATCTACAACAAAGACGAATTGGAAAATTTGACTGCCGATCAAGAGAAGGCACTTAAAAAGGCCATAGAGGCCGA
ACTGAAAAAACGAGGTACCCCATGA

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 11789.63 Da        Isoelectric Point: 10.3067

>AT63279 WP_024077333.1 NZ_CP015637:5497903-5498217 [Pseudomonas fluorescens]
MKKRDLFAELMQGVSDMTAHREGKITLRQSTVEDKPVPEVSAQEIVALRDKLHMSQSVFARSIRTSPGTLRNWEQDKSKP
NAQAALLIRLVEKFPDMVDRLATV

Download         Length: 315 bp

>AT63279 NZ_CP015637:5497903-5498217 [Pseudomonas fluorescens]
ATGAAAAAGCGCGATCTGTTTGCAGAGTTGATGCAGGGCGTTAGCGACATGACTGCTCACCGAGAAGGCAAAATCACTCT
TCGGCAAAGCACTGTGGAGGACAAGCCCGTTCCTGAGGTGTCAGCCCAAGAGATCGTGGCACTGCGGGATAAATTGCATA
TGTCACAGTCGGTGTTTGCCCGGAGCATACGGACGAGCCCCGGCACGCTGAGAAACTGGGAACAGGACAAATCCAAACCC
AATGCTCAGGCCGCACTCCTGATCAGGCTGGTTGAAAAGTTTCCGGATATGGTGGATCGATTGGCGACCGTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A855K4I9

Antitoxin

Source ID Structure
AlphaFold DB A0A855K566

References