Detailed information of TA system TA063266

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4819102..4819730
• Replicon: chromosome
• Accession: NZ_CP015613
• Organism: Serratia inhibens PRI-2C

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A1B1KWT6
• Locus tag: Q5A_RS22130
• Protein ID: WP_006320520.1
• Coordinates: 4819102..4819392 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: Q5A_RS22135
• Protein ID: WP_006320518.1
• Coordinates: 4819407..4819730 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Q5A_RS22120	4815664..4817685	+	2022	WP_006320527.1	NADPH-dependent 2,4-dienoyl-CoA reductase	-
Q5A_RS22125	4817732..4818871	-	1140	WP_006320522.1	23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG	-
Q5A_RS22130	4819102..4819392	+	291	WP_006320520.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
Q5A_RS22135	4819407..4819730	+	324	WP_006320518.1	HigA family addiction module antidote protein	Antitoxin
Q5A_RS22140	4819723..4820235	+	513	WP_006320516.1	M48 family metallopeptidase	-
Q5A_RS22145	4820336..4821316	+	981	WP_006320515.1	Gfo/Idh/MocA family oxidoreductase	-
Q5A_RS22150	4821318..4822049	+	732	WP_006320514.1	glutamine amidotransferase	-
Q5A_RS22155	4822309..4823280	+	972	WP_006328226.1	TerC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-94)

Antitoxin

• Cro/C1-type helix-turn-helix domain (24-71)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11762.46 Da
• Isoelectric Point: 10.0235

>T63266 WP_006320520.1 NZ_CP015613:4819102-4819392 [Serratia inhibens PRI-2C]
MIKSFRDRYLEKFYREGRRNRLIPSPLERQLARKLDILAAAQKECDLHHPTGHYYKRLSGPFQGWSSMWVNMQWRLMFQW
RHDAAEQIYLDPHQDT

Nucleotide

• Length: 291 bp

>T63266 NZ_CP015613:4819102-4819392 [Serratia inhibens PRI-2C]
ATGATTAAATCTTTCCGGGACAGATATCTGGAAAAATTCTATCGGGAAGGCCGGCGCAATCGTTTGATTCCTTCCCCCCT
TGAACGCCAATTGGCCAGAAAACTCGATATACTGGCCGCCGCACAAAAGGAATGTGATTTACATCATCCAACCGGTCATT
ATTACAAACGCCTGTCCGGCCCCTTTCAAGGCTGGTCCAGCATGTGGGTTAATATGCAATGGCGTTTAATGTTCCAATGG
CGCCATGATGCCGCAGAACAGATTTATCTGGACCCACATCAAGATACCTGA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 12186.88 Da
• Isoelectric Point: 6.7197

>AT63266 WP_006320518.1 NZ_CP015613:4819407-4819730 [Serratia inhibens PRI-2C]
MLETTTRKPTTVGEVLQEEYLTPLGIGQEDLAKVLGVTRATVNRLCNGHRRLSVAEANLFADLFETTADFWLNLQAAHDR
WEARQANMQRERLRPIMEILGRNVTHA

Nucleotide

• Length: 324 bp

>AT63266 NZ_CP015613:4819407-4819730 [Serratia inhibens PRI-2C]
ATGTTAGAAACCACAACCCGAAAACCCACGACGGTGGGGGAAGTGCTGCAAGAAGAGTATTTAACTCCGCTGGGTATCGG
CCAGGAAGATCTGGCGAAGGTTTTGGGGGTCACTCGCGCCACGGTAAACCGCTTATGCAACGGTCATCGCCGTCTCAGCG
TTGCGGAAGCCAATCTGTTTGCAGACTTGTTCGAAACCACGGCTGATTTTTGGCTAAACCTGCAAGCAGCCCACGACCGT
TGGGAAGCCAGACAGGCAAATATGCAACGTGAACGGCTGCGTCCGATTATGGAAATACTGGGGCGTAATGTTACCCATGC
ATGA

Structures

Toxin

• AlphaFold DB: A0A1B1KWT6