Detailed information of TA system TA063234

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 1706505..1707112
• Replicon: chromosome
• Accession: NZ_CP015600
• Organism: Pseudomonas antarctica strain PAMC 27494

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: A7J50_RS07810
• Protein ID: WP_064454872.1
• Coordinates: 1706505..1706819 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A172YY36
• Locus tag: A7J50_RS07815
• Protein ID: WP_064451284.1
• Coordinates: 1706819..1707112 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7J50_RS07785	1702190..1702600	-	411	WP_064451279.1	type II toxin-antitoxin system HicB family antitoxin	-
A7J50_RS07790	1702623..1702805	-	183	WP_064451280.1	type II toxin-antitoxin system HicA family toxin	-
A7J50_RS07795	1702973..1703866	-	894	WP_064451281.1	LysR family transcriptional regulator	-
A7J50_RS07800	1703962..1705125	+	1164	WP_064451282.1	MFS transporter	-
A7J50_RS07805	1705122..1706315	+	1194	WP_064451283.1	MFS transporter	-
A7J50_RS07810	1706505..1706819	+	315	WP_064454872.1	hypothetical protein	Toxin
A7J50_RS07815	1706819..1707112	+	294	WP_064451284.1	helix-turn-helix domain-containing protein	Antitoxin
A7J50_RS07820	1707132..1707335	-	204	WP_064451285.1	hypothetical protein	-
A7J50_RS07825	1707768..1708007	+	240	WP_064451286.1	hypothetical protein	-
A7J50_RS07830	1708089..1708394	-	306	WP_064451287.1	hypothetical protein	-
A7J50_RS07835	1708418..1708942	-	525	WP_064451288.1	DUF3833 domain-containing protein	-
A7J50_RS07845	1709427..1710311	-	885	WP_064451289.1	alpha/beta hydrolase	-
A7J50_RS07850	1710616..1711308	-	693	WP_064451290.1	pseudouridine synthase	-
A7J50_RS07855	1711308..1711520	-	213	WP_064451291.1	cysteine-rich CWC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-83)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11908.66 Da
• Isoelectric Point: 6.9798

>T63234 WP_064454872.1 NZ_CP015600:1706505-1706819 [Pseudomonas antarctica]
VIFVESSVFTEDLPALLSEESYGEFQEFLAENPTAGDVIQGTNGLRKIRWAVQGRGKRGGVRVIYYHLCMAYQIRLILIY
RKGIKDDLSAVEKSVLSEINRRWE

Nucleotide

• Length: 315 bp

>T63234 NZ_CP015600:1706505-1706819 [Pseudomonas antarctica]
GTGATATTTGTCGAATCATCCGTATTTACCGAGGACCTGCCAGCGCTACTGAGTGAGGAAAGCTACGGTGAGTTCCAGGA
ATTTCTTGCCGAAAACCCGACTGCTGGTGATGTCATCCAAGGGACCAATGGTCTGAGAAAGATCCGCTGGGCTGTGCAGG
GGAGGGGCAAGAGGGGTGGTGTCAGAGTGATCTATTACCACCTCTGCATGGCCTATCAAATTCGATTGATCCTGATCTAT
CGAAAGGGCATCAAAGATGATCTCTCTGCGGTGGAAAAGAGCGTGCTGAGCGAAATTAACCGAAGGTGGGAGTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10986.60 Da
• Isoelectric Point: 9.8595

>AT63234 WP_064451284.1 NZ_CP015600:1706819-1707112 [Pseudomonas antarctica]
MEKKLFNRLVESMTQMDEIARGERAPSREFFVDALKVKEIRQATGLSQARFAKAIDVPVGTLQNWEQGRREPEGPAKALL
RALYNDPDHVLAALGHR

Nucleotide

• Length: 294 bp

>AT63234 NZ_CP015600:1706819-1707112 [Pseudomonas antarctica]
ATGGAGAAAAAACTGTTCAATCGTCTGGTCGAAAGCATGACCCAGATGGACGAAATCGCACGGGGTGAGCGCGCCCCATC
CCGCGAATTTTTTGTTGATGCGTTGAAGGTAAAAGAGATTCGACAAGCCACAGGACTTTCACAAGCACGCTTTGCGAAGG
CAATTGATGTGCCGGTGGGAACGCTTCAAAACTGGGAGCAGGGGCGACGTGAACCAGAGGGGCCGGCAAAGGCATTATTG
CGTGCCTTATACAATGATCCGGATCACGTATTGGCCGCTCTGGGGCATCGATAA

Structures

Antitoxin

• AlphaFold DB: A0A172YY36