Detailed information of TA system TA063136

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2255..2525
• Replicon: plasmid unnamed 3
• Accession: NZ_CP015503
• Organism: Klebsiella pneumoniae strain SKGH01

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: A7B01_RS31865
• Protein ID: WP_001312861.1
• Coordinates: 2367..2525 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	2255..2318 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7B01_RS29225	937..1371	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
A7B01_RS29230	1368..2087	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	2099..2323	+	225	NuclAT_0	-	-
-	2099..2323	+	225	NuclAT_0	-	-
-	2099..2323	+	225	NuclAT_0	-	-
-	2099..2323	+	225	NuclAT_0	-	-
-	2255..2318	-	64	-	-	Antitoxin
A7B01_RS31865	2367..2525	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
A7B01_RS33755	2763..3140	-	378	Protein_4	hypothetical protein	-
A7B01_RS29240	3440..3736	+	297	WP_001272251.1	hypothetical protein	-
A7B01_RS29245	3847..4668	+	822	WP_001234445.1	DUF945 domain-containing protein	-
A7B01_RS29250	4965..5567	-	603	WP_000243713.1	transglycosylase SLT domain-containing protein	-
A7B01_RS29255	5890..6273	+	384	WP_001354030.1	relaxosome protein TraM	-
A7B01_RS29260	6467..7138	+	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
A7B01_RS29265	7275..7502	+	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	rmtF / aadA2 / dfrA12	-	1..84941	84941

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T63136 WP_001312861.1 NZ_CP015503:2367-2525 [Klebsiella pneumoniae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T63136 NZ_CP015503:2367-2525 [Klebsiella pneumoniae]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 64 bp

>AT63136 NZ_CP015503:c2318-2255 [Klebsiella pneumoniae]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin