Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 4737062..4737771 Replicon chromosome
Accession NZ_CP015495
Organism Mycobacterium avium subsp. paratuberculosis strain MAP/TANUVAS/TN/India/2008

Toxin (Protein)


Gene name HigB2 Uniprot ID X7V9P5
Locus tag A0V42_RS21900 Protein ID WP_003873387.1
Coordinates 4737367..4737771 (-) Length 135 a.a.

Antitoxin (Protein)


Gene name HigA2 Uniprot ID Q73S10
Locus tag A0V42_RS21895 Protein ID WP_003873388.1
Coordinates 4737062..4737370 (-) Length 103 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A0V42_RS21880 4732947..4733249 + 303 WP_003873391.1 co-chaperone GroES -
A0V42_RS21885 4733338..4734954 + 1617 WP_003873390.1 chaperonin GroEL -
A0V42_RS21890 4735049..4736575 + 1527 WP_003879517.1 adenylate/guanylate cyclase domain-containing protein -
A0V42_RS21895 4737062..4737370 - 309 WP_003873388.1 helix-turn-helix transcriptional regulator Antitoxin
A0V42_RS21900 4737367..4737771 - 405 WP_003873387.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
A0V42_RS21905 4738011..4738703 + 693 WP_010950286.1 DUF4352 domain-containing protein -
A0V42_RS21910 4738758..4739957 + 1200 WP_010950287.1 FAD-dependent oxidoreductase -
A0V42_RS21915 4740114..4740878 - 765 WP_003874401.1 superoxide dismutase family protein -
A0V42_RS21920 4740921..4741229 - 309 WP_003874400.1 WhiB family transcriptional regulator -
A0V42_RS21925 4741627..4742457 + 831 WP_003874399.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-109)

Antitoxin

HigA2-like, helix-turn-helix domain

(31-91)


Sequences

Toxin        


Download         Length: 135 a.a.        Molecular weight: 15503.82 Da        Isoelectric Point: 10.3815

>T63112 WP_003873387.1 NZ_CP015495:c4737771-4737367 [Mycobacterium avium subsp. paratuberculosis]
VRKWEVDLTLIEAWMDALDDEEYDNLIAALEQLEEHGPITRRPFVDTLEGSRHPNMKELRPRPTKAGAHIRVLFAFDTRS
RAIMLIAGDKAGNWSKWYAKHIPIADELFDAHQKRLHKAAAKATNRKPRKGKKR

Download         Length: 405 bp

>T63112 NZ_CP015495:c4737771-4737367 [Mycobacterium avium subsp. paratuberculosis]
GTGCGGAAATGGGAGGTTGACCTGACTCTCATCGAAGCCTGGATGGATGCCCTCGATGACGAGGAGTACGACAACTTGAT
AGCAGCGCTCGAGCAGTTGGAGGAACACGGTCCAATTACCCGGCGGCCGTTTGTGGACACCCTTGAAGGGTCAAGGCACC
CGAACATGAAGGAGCTCCGTCCGCGCCCCACGAAAGCTGGAGCCCACATTCGCGTGCTATTCGCCTTCGATACCCGGTCG
CGGGCGATCATGTTGATCGCTGGAGACAAGGCGGGCAATTGGTCCAAGTGGTATGCCAAGCACATTCCCATTGCCGACGA
GCTCTTCGATGCTCACCAAAAGCGTTTACACAAGGCAGCAGCCAAAGCCACCAATCGGAAACCAAGGAAGGGAAAGAAAC
GATGA

Antitoxin


Download         Length: 103 a.a.        Molecular weight: 11445.94 Da        Isoelectric Point: 10.0801

>AT63112 WP_003873388.1 NZ_CP015495:c4737370-4737062 [Mycobacterium avium subsp. paratuberculosis]
MTNLDDMRRRRPGNRTRIDAIKAEMDREVAQYRLRELREAAGYTQTTLAAAIGVGQNRVSQMEHGDLGTSRVDTLRKYVE
ATGGELEVSVKRPDGSRVLLSL

Download         Length: 309 bp

>AT63112 NZ_CP015495:c4737370-4737062 [Mycobacterium avium subsp. paratuberculosis]
ATGACCAATCTTGATGACATGCGCCGTCGCCGTCCCGGCAACCGGACCCGTATCGACGCGATCAAGGCGGAGATGGACCG
CGAGGTTGCCCAGTACCGTCTCCGCGAACTGCGCGAGGCTGCCGGCTACACGCAGACGACTCTCGCTGCGGCTATTGGCG
TCGGGCAGAACCGCGTCTCGCAGATGGAACATGGCGATCTGGGCACGAGCCGGGTCGACACTCTCCGCAAGTACGTGGAA
GCGACTGGGGGTGAGCTGGAAGTGTCCGTCAAGCGTCCGGACGGGTCACGGGTTCTCCTCAGCCTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB X7V9P5

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4U5M0

References