Detailed information of TA system TA063037

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 70291..71015
• Replicon: plasmid pCR14_4
• Accession: NZ_CP015396
• Organism: Klebsiella pneumoniae strain CR14

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A2J4YLV3
• Locus tag: A6P37_RS30675
• Protein ID: WP_023292165.1
• Coordinates: 70291..70602 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: A6P37_RS30680
• Protein ID: WP_020801939.1
• Coordinates: 70599..71015 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A6P37_RS30625	65650..65994	+	345	Protein_67	conjugation system SOS inhibitor PsiB	-
A6P37_RS30635	66101..67309	+	1209	WP_001339197.1	IS4-like element ISVsa5 family transposase	-
A6P37_RS30640	67407..68129	+	723	WP_020804573.1	plasmid SOS inhibition protein A	-
A6P37_RS30645	68487..68759	+	273	WP_042922555.1	hypothetical protein	-
A6P37_RS30650	68756..69106	+	351	WP_004152758.1	hypothetical protein	-
A6P37_RS30665	69743..69949	+	207	WP_042922554.1	hypothetical protein	-
A6P37_RS30670	69960..70184	+	225	WP_042922612.1	hypothetical protein	-
A6P37_RS30675	70291..70602	+	312	WP_023292165.1	type II toxin-antitoxin system HigB family toxin	Toxin
A6P37_RS30680	70599..71015	+	417	WP_020801939.1	helix-turn-helix domain-containing protein	Antitoxin
A6P37_RS30695	71737..72117	+	381	WP_020802391.1	hypothetical protein	-
A6P37_RS30700	72184..72531	+	348	WP_020804846.1	hypothetical protein	-
A6P37_RS30705	72626..72772	+	147	WP_004152750.1	hypothetical protein	-
A6P37_RS30710	72821..73654	+	834	WP_042922549.1	type I restriction-modification system subunit M	-
A6P37_RS30730	74480..75301	+	822	WP_020804822.1	DUF945 domain-containing protein	-
A6P37_RS30735	75361..75669	+	309	WP_071599372.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(6')-Ib	-	1..110092	110092

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-133)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12497.32 Da
• Isoelectric Point: 9.9242

>T63037 WP_023292165.1 NZ_CP015396:70291-70602 [Klebsiella pneumoniae]
VHVISRAPFDEAARHYPNDAAAIDDTYRVLKRVVAKKPDELKRYFTSLDRMKYREKWWVIDIGGNNLRMMFFADFERGKI
FVKHITTHAEYDRLTKYYREHKE

Nucleotide

• Length: 312 bp

>T63037 NZ_CP015396:70291-70602 [Klebsiella pneumoniae]
GTGCATGTTATATCACGAGCCCCTTTTGATGAGGCGGCGAGACACTATCCGAATGATGCTGCGGCCATCGATGACACCTA
TCGTGTACTAAAGCGGGTTGTCGCAAAAAAACCGGATGAGCTAAAGCGATACTTTACTAGTCTGGATCGCATGAAATACC
GGGAAAAATGGTGGGTTATCGATATCGGTGGTAATAACCTTAGAATGATGTTTTTTGCTGATTTTGAGCGCGGAAAGATA
TTCGTCAAACACATAACCACCCATGCTGAATATGATCGCTTAACGAAATATTATCGGGAGCACAAAGAATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15380.49 Da
• Isoelectric Point: 4.5542

>AT63037 WP_020801939.1 NZ_CP015396:70599-71015 [Klebsiella pneumoniae]
MIFSDAIKAANDLASIVPLLGGSSSRKDYEEALKLVEYLLEHDPDSPLVDMLTARIDAWEDNAVEFEEFNTRIEAGKNGV
SLLRVLMQQHGLSQSDFENEIGKKSLVSRILSGERSLTLDHMRALANRFQIPVSMFVG

Nucleotide

• Length: 417 bp

>AT63037 NZ_CP015396:70599-71015 [Klebsiella pneumoniae]
ATGATTTTTAGCGATGCCATTAAAGCGGCGAATGACCTGGCGAGTATTGTCCCTCTGCTGGGTGGCAGCTCGTCCCGCAA
AGATTACGAAGAGGCGCTTAAACTCGTTGAATATCTTCTGGAACATGACCCGGACAGCCCGCTGGTTGATATGCTGACTG
CCCGCATCGATGCCTGGGAAGATAACGCGGTGGAGTTTGAAGAATTTAATACGCGCATCGAAGCAGGCAAAAACGGTGTA
TCGTTGCTGCGGGTTCTTATGCAGCAACACGGGCTTTCACAGTCCGATTTTGAAAACGAGATAGGCAAAAAATCGCTCGT
CAGCCGGATCCTCAGTGGTGAACGCAGTCTGACCCTTGATCACATGCGGGCGCTGGCAAATCGATTTCAAATCCCGGTCT
CGATGTTTGTTGGCTGA

Structures

Toxin

• AlphaFold DB: A0A2J4YLV3