Detailed information of TA system TA063035

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 13573..14230
• Replicon: plasmid pCR14_2
• Accession: NZ_CP015394
• Organism: Klebsiella pneumoniae strain CR14

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: A6P37_RS28680
• Protein ID: WP_000270043.1
• Coordinates: 13880..14230 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: A6P37_RS28675
• Protein ID: WP_000124640.1
• Coordinates: 13573..13875 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A6P37_RS28625	9198..9626	+	429	WP_000591074.1	hypothetical protein	-
A6P37_RS28630	9683..10042	+	360	WP_000422768.1	hypothetical protein	-
A6P37_RS28635	10042..10488	+	447	WP_000919345.1	Fe3+-siderophore ABC transporter permease	-
A6P37_RS28640	10485..11003	+	519	WP_000210756.1	nitrite reductase	-
A6P37_RS28645	11003..11233	+	231	WP_000972663.1	hypothetical protein	-
A6P37_RS28650	11220..12077	+	858	WP_001167032.1	hypothetical protein	-
A6P37_RS28660	12308..12835	+	528	WP_001236377.1	thermonuclease family protein	-
A6P37_RS28665	12893..13165	+	273	WP_001043047.1	HU family DNA-binding protein	-
A6P37_RS28670	13253..13546	+	294	WP_001239997.1	chromosome segregation protein ParM	-
A6P37_RS28675	13573..13875	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
A6P37_RS28680	13880..14230	-	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A6P37_RS28685	14393..14941	+	549	WP_001061574.1	transcriptional regulator	-
A6P37_RS28695	15282..15476	+	195	WP_000343597.1	hypothetical protein	-
A6P37_RS28700	15487..15858	+	372	WP_000516916.1	hypothetical protein	-
A6P37_RS28705	15851..16321	+	471	WP_001281821.1	hypothetical protein	-
A6P37_RS28710	16336..16671	-	336	WP_000683476.1	hypothetical protein	-
A6P37_RS28715	16768..17256	+	489	WP_001273096.1	DUF1643 domain-containing protein	-
A6P37_RS28720	17259..17756	+	498	WP_000062185.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaOXA-2 / qacE / sul1 / blaCTX-M-2 / blaTEM-1B / aac(3)-IIa	-	1..154343	154343

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T63035 WP_000270043.1 NZ_CP015394:c14230-13880 [Klebsiella pneumoniae]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T63035 NZ_CP015394:c14230-13880 [Klebsiella pneumoniae]
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT63035 WP_000124640.1 NZ_CP015394:c13875-13573 [Klebsiella pneumoniae]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT63035 NZ_CP015394:c13875-13573 [Klebsiella pneumoniae]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9