Detailed information of TA system TA063012

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 44429..44698
• Replicon: plasmid pNY9_3
• Accession: NZ_CP015388
• Organism: Klebsiella pneumoniae strain NY9

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: A6P56_RS34210
• Protein ID: WP_001312861.1
• Coordinates: 44540..44698 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	44429..44494 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A6P56_RS30625	40139..40666	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
A6P56_RS30630	40724..40957	+	234	WP_000006003.1	DUF905 family protein	-
A6P56_RS30635	41018..43041	+	2024	Protein_48	ParB/RepB/Spo0J family partition protein	-
A6P56_RS30640	43110..43544	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
A6P56_RS30645	43541..44260	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	44272..44496	+	225	NuclAT_0	-	-
-	44272..44496	+	225	NuclAT_0	-	-
-	44272..44496	+	225	NuclAT_0	-	-
-	44272..44496	+	225	NuclAT_0	-	-
-	44429..44494	+	66	-	-	Antitoxin
A6P56_RS34210	44540..44698	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
A6P56_RS30655	45056..45481	+	426	WP_000422741.1	IS66 family insertion sequence hypothetical protein	-
A6P56_RS30660	45478..45828	+	351	WP_000624722.1	IS66 family insertion sequence element accessory protein TnpB	-
A6P56_RS30665	45859..47472	+	1614	WP_000080200.1	IS66-like element ISEc23 family transposase	-
A6P56_RS34445	47557..47761	-	205	Protein_55	pilus protein	-
A6P56_RS30685	48153..48449	+	297	WP_001272251.1	hypothetical protein	-
A6P56_RS30690	48560..49381	+	822	WP_001234445.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / aac(6')-Ib-cr / blaOXA-1 / blaCTX-M-15 / blaTEM-1B	-	1..89067	89067

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T63012 WP_001312861.1 NZ_CP015388:44540-44698 [Klebsiella pneumoniae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T63012 NZ_CP015388:44540-44698 [Klebsiella pneumoniae]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT63012 NZ_CP015388:44429-44494 [Klebsiella pneumoniae]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin