Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1018686..1019281 Replicon chromosome
Accession NZ_CP015377
Organism Pseudomonas aeruginosa strain BAMCPA07-48

Toxin (Protein)


Gene name higB Uniprot ID V6ALY3
Locus tag A6R75_RS33050 Protein ID WP_003113526.1
Coordinates 1019003..1019281 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0S3KUN4
Locus tag A6R75_RS04890 Protein ID WP_003111575.1
Coordinates 1018686..1018991 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A6R75_RS04855 1013838..1014686 + 849 WP_023092961.1 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase -
A6R75_RS04865 1014853..1015794 + 942 WP_003099281.1 ribose-phosphate pyrophosphokinase -
A6R75_RS04870 1015911..1016525 + 615 WP_003095013.1 50S ribosomal protein L25/general stress protein Ctc -
A6R75_RS04875 1016567..1017151 + 585 WP_003099278.1 aminoacyl-tRNA hydrolase -
A6R75_RS04880 1017192..1018292 + 1101 WP_003099270.1 redox-regulated ATPase YchF -
A6R75_RS04890 1018686..1018991 - 306 WP_003111575.1 HigA family addiction module antidote protein Antitoxin
A6R75_RS33050 1019003..1019281 - 279 WP_003113526.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
A6R75_RS04895 1019610..1021838 + 2229 WP_021264080.1 TonB-dependent receptor -
A6R75_RS04900 1021908..1022555 - 648 WP_003095021.1 carbonate dehydratase -
A6R75_RS04905 1022617..1023855 - 1239 WP_063456069.1 C69 family dipeptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(5-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(6-82)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10646.19 Da        Isoelectric Point: 7.8937

>T62938 WP_003113526.1 NZ_CP015377:c1019281-1019003 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Download         Length: 279 bp

>T62938 NZ_CP015377:c1019281-1019003 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACTTGCGCTCTCCACCTGGAAACCGCTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11205.72 Da        Isoelectric Point: 4.4475

>AT62938 WP_003111575.1 NZ_CP015377:c1018991-1018686 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGEQIEHEIEPLLAQG

Download         Length: 306 bp

>AT62938 NZ_CP015377:c1018991-1018686 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAATGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTACTCGTTAGCA
ACTGCTTATGCGGCGAATGGCGAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCAGGGATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V6ALY3

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3KUN4

References