Detailed information of TA system TA062888

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): HipBST/HipA(toxin)
• Location: 2725152..2726389
• Replicon: chromosome
• Accession: NZ_CP015344
• Organism: Legionella pneumophila strain D-7630

Toxin (Protein)

• Gene name: HipT
• Uniprot ID: -
• Locus tag: A5479_RS12165
• Protein ID: WP_027224428.1
• Coordinates: 2725457..2726389 (+)
• Length: 311 a.a.

Antitoxin (Protein)

• Gene name: HipS
• Uniprot ID: -
• Locus tag: A5479_RS12160
• Protein ID: WP_027224429.1
• Coordinates: 2725152..2725460 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A5479_RS12135	2720838..2721035	-	198	WP_014842401.1	hypothetical protein	-
A5479_RS12140	2721055..2722281	-	1227	WP_027224432.1	MFS transporter	-
A5479_RS12145	2722916..2723389	+	474	WP_027224431.1	hypothetical protein	-
A5479_RS12150	2723568..2724485	+	918	WP_027224430.1	LysR family transcriptional regulator	-
A5479_RS12155	2724928..2725155	+	228	WP_013101792.1	helix-turn-helix transcriptional regulator	-
A5479_RS12160	2725152..2725460	+	309	WP_027224429.1	HipA N-terminal domain-containing protein	Antitoxin
A5479_RS12165	2725457..2726389	+	933	WP_027224428.1	HipA domain-containing protein	Toxin
A5479_RS12170	2726518..2729085	+	2568	WP_027224427.1	magnesium-translocating P-type ATPase	-
A5479_RS12175	2729120..2730064	+	945	WP_027224426.1	flippase-like domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (53-279)

Antitoxin

• HipA, N-terminal subdomain 1 (5-98)

Sequences

Toxin

Protein

• Length: 311 a.a.
• Molecular weight: 35780.41 Da
• Isoelectric Point: 9.5904

>T62888 WP_027224428.1 NZ_CP015344:2725457-2726389 [Legionella pneumophila]
MKRCPITYEKISDQENYSQRGLRLLSPQLKNLSPLDLSADEQRQEAIARVGKMSIQGVQKKLSAKLKIKEGSFEIVDQNG
HYILKPQSDIYPELPENEAITMTLAKTIGLEVPVHGLVYSKDNSLTYFIKRFDRIGHNKKLALEDFAQLSGEDRHTKYKS
SMEKVIAIIEQFCTFPKIEFVKLFKLTLFNFLVGNEDMHLKNFSLITRDRKISISPAYDLLNSTIAQKNTKEEIALPLKG
KKNNLTKSDFLNYFAVEKLGLNQNVINGIAQEFHQAIPEWRELISFSFLSQPMQEKYLQLLDQRCKRLNF

Nucleotide

• Length: 933 bp

>T62888 NZ_CP015344:2725457-2726389 [Legionella pneumophila]
ATGAAACGCTGCCCGATTACTTATGAAAAAATCAGTGACCAAGAAAACTATTCACAACGTGGATTGCGCTTGCTTTCTCC
TCAATTAAAAAACCTGAGCCCATTAGATTTAAGTGCTGATGAGCAGCGTCAGGAAGCAATTGCTCGAGTAGGAAAGATGT
CTATTCAAGGTGTCCAAAAGAAACTAAGCGCAAAACTAAAGATTAAAGAGGGATCTTTTGAAATCGTGGATCAAAATGGT
CACTATATTTTAAAACCACAAAGTGATATTTATCCAGAATTGCCGGAAAACGAAGCCATTACCATGACCCTTGCAAAGAC
CATTGGCCTTGAAGTGCCTGTTCATGGTTTAGTGTACTCTAAAGATAACAGTTTGACTTACTTCATTAAACGGTTTGATA
GAATAGGCCATAATAAAAAATTAGCTTTAGAAGATTTTGCACAGCTATCGGGTGAAGATCGACATACAAAATATAAAAGT
TCTATGGAAAAAGTGATTGCAATCATAGAGCAGTTTTGCACGTTCCCCAAAATTGAATTCGTGAAATTATTTAAGTTGAC
ATTGTTTAACTTCCTGGTCGGCAATGAAGACATGCACCTAAAAAATTTTTCTCTAATTACTAGGGATAGAAAAATTTCCA
TATCACCAGCATACGATTTACTTAACTCAACTATCGCTCAAAAAAACACAAAAGAAGAAATCGCATTGCCCCTCAAAGGG
AAAAAAAATAATTTAACAAAGAGTGATTTTCTTAACTATTTTGCCGTTGAGAAATTAGGATTAAATCAGAACGTCATCAA
TGGGATCGCACAAGAGTTTCATCAGGCAATACCAGAATGGCGAGAGTTGATTAGTTTCAGTTTTTTATCTCAACCGATGC
AGGAAAAATATCTCCAATTGCTCGATCAACGATGCAAGAGATTAAATTTTTGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11586.38 Da
• Isoelectric Point: 4.5859

>AT62888 WP_027224429.1 NZ_CP015344:2725152-2725460 [Legionella pneumophila]
MRKAYVSVNGIKAGILEELQSGKYQFSYFDDYHGAPVSLTMPLTNKVYDFDVFPPFFEGLLPEGIMLEALLRKYKIDKND
YFGQLIIVGQDVVGAVTIEEIR

Nucleotide

• Length: 309 bp

>AT62888 NZ_CP015344:2725152-2725460 [Legionella pneumophila]
ATGAGAAAAGCATACGTATCAGTGAATGGCATCAAGGCTGGAATATTAGAAGAATTACAAAGCGGAAAATATCAATTCAG
TTACTTTGATGATTATCACGGGGCACCTGTTTCTCTCACAATGCCGTTAACCAATAAGGTCTATGATTTTGATGTATTTC
CCCCTTTTTTTGAAGGGTTACTTCCTGAAGGCATTATGCTTGAAGCACTATTACGCAAATATAAAATCGATAAAAATGAT
TATTTTGGCCAATTGATAATAGTTGGCCAGGATGTCGTAGGCGCAGTGACTATTGAGGAAATAAGATGA