Detailed information of TA system TA062851

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 3376693..3377320
• Replicon: chromosome
• Accession: NZ_CP015281
• Organism: Photorhabdus laumondii subsp. laumondii strain DSPV002N

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q7N374
• Locus tag: A4R40_RS14645
• Protein ID: WP_011147065.1
• Coordinates: 3376693..3377022 (+)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: A4R40_RS14650
• Protein ID: WP_041380121.1
• Coordinates: 3377012..3377320 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A4R40_RS14630	3373240..3373872	+	633	WP_011147062.1	SDR family oxidoreductase	-
A4R40_RS14635	3373917..3374234	-	318	WP_011147063.1	DUF496 family protein	-
A4R40_RS14640	3375436..3375666	+	231	WP_041380119.1	hypothetical protein	-
A4R40_RS14645	3376693..3377022	+	330	WP_011147065.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A4R40_RS14650	3377012..3377320	+	309	WP_041380121.1	helix-turn-helix domain-containing protein	Antitoxin
A4R40_RS14655	3377476..3378906	+	1431	WP_011147067.1	exodeoxyribonuclease I	-
A4R40_RS14660	3379034..3380749	-	1716	WP_011147068.1	D-lactate dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (15-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (44-97)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12899.94 Da
• Isoelectric Point: 9.7776

>T62851 WP_011147065.1 NZ_CP015281:3376693-3377022 [Photorhabdus laumondii subsp. laumondii]
MKYTIYYYSTEVENEILALPDTLQARYIRYSERMREHGANLGEPHTEAFGNGLFELRLKGAEGIARVFYCTMIGHRIVML
HSFVKKTQKTPLKERRKAEVRMKEVKNDW

Nucleotide

• Length: 330 bp

>T62851 NZ_CP015281:3376693-3377022 [Photorhabdus laumondii subsp. laumondii]
ATGAAATATACTATCTATTACTATAGCACCGAGGTGGAGAACGAGATCCTTGCTCTCCCTGACACACTTCAAGCCCGTTA
CATCAGGTATTCCGAAAGAATGCGAGAACATGGTGCCAACCTCGGAGAACCACACACAGAAGCGTTTGGTAATGGGTTGT
TTGAACTCAGACTCAAAGGGGCTGAAGGAATTGCTCGCGTATTCTATTGCACAATGATTGGGCACCGTATAGTGATGCTG
CACAGCTTCGTGAAGAAAACACAAAAGACCCCACTTAAAGAACGCAGGAAAGCCGAAGTCAGAATGAAAGAGGTAAAAAA
TGACTGGTAA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11170.03 Da
• Isoelectric Point: 10.2817

>AT62851 WP_041380121.1 NZ_CP015281:3377012-3377320 [Photorhabdus laumondii subsp. laumondii]
MTGKYTPPTMTHDEMVAKMLSNPAVKAEYEKLEQEFALLDELLAARKKAGLTQAQVAERMGTKATAITRLESRLASGTQG
PSYNTLKKYAAAVGKKLQIRLV

Nucleotide

• Length: 309 bp

>AT62851 NZ_CP015281:3377012-3377320 [Photorhabdus laumondii subsp. laumondii]
ATGACTGGTAAATATACCCCTCCAACCATGACACACGATGAAATGGTTGCAAAAATGTTGTCAAATCCGGCTGTAAAAGC
TGAATACGAGAAACTGGAACAAGAATTTGCATTGCTTGATGAACTGCTAGCTGCACGCAAAAAGGCCGGGCTAACACAAG
CGCAAGTAGCTGAACGCATGGGAACCAAAGCAACAGCCATCACACGGCTTGAAAGCCGGCTAGCTTCTGGCACACAAGGG
CCTTCGTATAATACATTGAAGAAATATGCAGCAGCTGTCGGTAAAAAATTACAGATACGCCTAGTATGA

Structures

Toxin

• AlphaFold DB: A0A6L9JI68