Detailed information of TA system TA062850

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2984443..2985047
• Replicon: chromosome
• Accession: NZ_CP015281
• Organism: Photorhabdus laumondii subsp. laumondii strain DSPV002N

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q7N418
• Locus tag: A4R40_RS13065
• Protein ID: WP_011146760.1
• Coordinates: 2984736..2985047 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: A4R40_RS13060
• Protein ID: WP_109791601.1
• Coordinates: 2984443..2984733 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A4R40_RS13040	2980403..2980852	-	450	WP_011146754.1	phage tail protein	-
A4R40_RS13045	2981559..2982152	+	594	WP_011146755.1	winged helix-turn-helix domain-containing protein	-
A4R40_RS13050	2982681..2983025	+	345	WP_011146757.1	hypothetical protein	-
A4R40_RS13055	2983487..2984080	+	594	WP_109791600.1	winged helix-turn-helix domain-containing protein	-
A4R40_RS13060	2984443..2984733	-	291	WP_109791601.1	helix-turn-helix domain-containing protein	Antitoxin
A4R40_RS13065	2984736..2985047	-	312	WP_011146760.1	hypothetical protein	Toxin
A4R40_RS13070	2985501..2986271	-	771	WP_011146762.1	DUF2829 domain-containing protein	-
A4R40_RS13075	2986640..2987478	-	839	Protein_2495	IS5 family transposase	-
A4R40_RS13080	2987528..2987785	-	258	WP_011146765.1	type II toxin-antitoxin system YafO family toxin	-
A4R40_RS13085	2987894..2988466	-	573	WP_125026236.1	hypothetical protein	-
A4R40_RS13090	2988555..2988806	-	252	WP_011146767.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-87)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 11976.74 Da
• Isoelectric Point: 8.0254

>T62850 WP_011146760.1 NZ_CP015281:c2985047-2984736 [Photorhabdus laumondii subsp. laumondii]
MIFIETHIFTEDCKELLSDDEYREFQQYLADNPIAGDVIQHTGGLRKVRWASGSKGKRGGVRIIYYYKVDVSHIRLLLIY
KKGIKDDLSESEKKVLRALNEGW

Nucleotide

• Length: 312 bp

>T62850 NZ_CP015281:c2985047-2984736 [Photorhabdus laumondii subsp. laumondii]
ATGATATTTATTGAGACCCATATCTTCACTGAAGATTGTAAAGAGTTGTTAAGTGACGATGAATATCGTGAATTTCAGCA
ATATCTCGCAGACAATCCTATTGCTGGTGATGTTATCCAACACACTGGTGGCTTACGTAAAGTCAGATGGGCATCTGGGA
GTAAAGGTAAGCGGGGTGGTGTGCGCATTATTTATTACTATAAAGTAGATGTCTCACACATCAGATTGTTACTGATTTAC
AAAAAAGGCATCAAGGATGACCTTAGTGAATCAGAAAAGAAAGTCTTGAGAGCACTAAATGAGGGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10664.37 Da
• Isoelectric Point: 10.9696

>AT62850 WP_109791601.1 NZ_CP015281:c2984733-2984443 [Photorhabdus laumondii subsp. laumondii]
MDSKLFSRLTESMTQMNEIINGERAASRETRVEAVKVKNIRQATGLTQTGFAKLISVNVGTLRNWEQGRREPTGPAKALL
KAIEKDPVHVLKALSM

Nucleotide

• Length: 291 bp

>AT62850 NZ_CP015281:c2984733-2984443 [Photorhabdus laumondii subsp. laumondii]
ATGGATAGTAAATTATTTTCCAGGCTAACTGAAAGCATGACACAGATGAACGAAATTATTAATGGCGAACGTGCTGCCTC
TCGTGAAACTCGCGTTGAAGCAGTAAAAGTAAAGAATATTCGCCAGGCAACCGGTTTAACTCAGACCGGTTTTGCTAAGT
TGATTTCCGTTAATGTTGGAACTCTGCGCAACTGGGAACAGGGAAGGCGTGAGCCAACCGGACCAGCAAAAGCCCTGTTG
AAGGCAATAGAGAAAGATCCGGTTCATGTTTTAAAGGCGTTATCTATGTAA

Structures

Toxin

• AlphaFold DB: A0A6L9JR19