Detailed information of TA system TA062783

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 39958..40212
• Replicon: plasmid pRM7190-2
• Accession: NZ_CP015246
• Organism: Escherichia coli O91 str. RM7190

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: I51_RS25680
• Protein ID: WP_032215358.1
• Coordinates: 40006..40212 (+)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	39958..40019 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I51_RS25645	35409..36149	+	741	WP_000206160.1	conjugal transfer pilus acetylase TraX	-
I51_RS25650	36208..37068	+	861	WP_000868985.1	alpha/beta hydrolase	-
I51_RS25655	37171..37734	+	564	WP_000139382.1	fertility inhibition protein FinO	-
I51_RS25660	37888..38091	+	204	WP_001327131.1	hypothetical protein	-
I51_RS25665	38836..39297	+	462	WP_000417388.1	thermonuclease family protein	-
I51_RS25670	39400..39783	+	384	WP_001109066.1	hypothetical protein	-
-	39958..40019	-	62	NuclAT_0	-	Antitoxin
-	39958..40019	-	62	NuclAT_0	-	Antitoxin
-	39958..40019	-	62	NuclAT_0	-	Antitoxin
-	39958..40019	-	62	NuclAT_0	-	Antitoxin
I51_RS25680	40006..40212	+	207	WP_032215358.1	type I toxin-antitoxin system Hok family toxin	Toxin
I51_RS25685	40496..40744	+	249	WP_000083842.1	replication regulatory protein RepA	-
I51_RS25695	40990..41064	+	75	WP_001367777.1	RepA leader peptide Tap	-
I51_RS25700	41057..41914	+	858	WP_000130938.1	incFII family plasmid replication initiator RepA	-
I51_RS25720	42887..43126	+	240	WP_000859018.1	MarR family transcriptional regulator	-
I51_RS25730	43469..43884	-	416	Protein_42	transposase	-
I51_RS25735	44167..44589	+	423	WP_001212724.1	Exc2 family lipoprotein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyC / hlyA / hlyB / hlyD	1..118513	118513
	-	flank	IS/Tn	-	-	43457..43771	314

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7778.27 Da
• Isoelectric Point: 8.8807

>T62783 WP_032215358.1 NZ_CP015246:40006-40212 [Escherichia coli O91 str. RM7190]
MKYLNTTDCSLFLAERSKFMTKYALIGVLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T62783 NZ_CP015246:40006-40212 [Escherichia coli O91 str. RM7190]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCCTTGCAGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GGTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAGCTGAATATTCACAGGG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT62783 NZ_CP015246:c40019-39958 [Escherichia coli O91 str. RM7190]
TTAAGGTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin