Detailed information of TA system TA062775

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3049348..3049950
• Replicon: chromosome
• Accession: NZ_CP015244
• Organism: Escherichia coli O91 str. RM7190

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: I51_RS15845
• Protein ID: WP_000897305.1
• Coordinates: 3049639..3049950 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I51_RS15840
• Protein ID: WP_000356397.1
• Coordinates: 3049348..3049638 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I51_RS15810	3045293..3046195	+	903	WP_021548410.1	formate dehydrogenase O subunit beta	-
I51_RS15815	3046192..3046827	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
I51_RS15820	3046824..3047753	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
I51_RS15825	3048083..3048325	-	243	WP_001087409.1	hypothetical protein	-
I51_RS15830	3048544..3048762	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
I51_RS15840	3049348..3049638	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
I51_RS15845	3049639..3049950	-	312	WP_000897305.1	hypothetical protein	Toxin
I51_RS15850	3050179..3051087	+	909	WP_001367979.1	alpha/beta hydrolase	-
I51_RS15855	3051151..3052092	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
I51_RS15860	3052137..3052574	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
I51_RS15865	3052571..3053443	-	873	WP_000920762.1	virulence factor BrkB family protein	-
I51_RS15870	3053437..3054036	-	600	WP_001295269.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T62775 WP_000897305.1 NZ_CP015244:c3049950-3049639 [Escherichia coli O91 str. RM7190]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T62775 NZ_CP015244:c3049950-3049639 [Escherichia coli O91 str. RM7190]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT62775 WP_000356397.1 NZ_CP015244:c3049638-3049348 [Escherichia coli O91 str. RM7190]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT62775 NZ_CP015244:c3049638-3049348 [Escherichia coli O91 str. RM7190]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7