Detailed information of TA system TA062745

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3251336..3252030
• Replicon: chromosome
• Accession: NZ_CP015241
• Organism: Escherichia coli strain 2013C-4465

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: A5955_RS15950
• Protein ID: WP_001263495.1
• Coordinates: 3251632..3252030 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: A5955_RS15945
• Protein ID: WP_000554757.1
• Coordinates: 3251336..3251629 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A5955_RS31105	3246959..3247456	+	498	Protein_3001	REP-associated tyrosine transposase RayT	-
A5955_RS15930	3247689..3249401	-	1713	Protein_3002	flagellar biosynthesis protein FlhA	-
A5955_RS15935	3249373..3250158	+	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
A5955_RS15940	3250229..3251284	+	1056	WP_001226186.1	DNA polymerase IV	-
A5955_RS15945	3251336..3251629	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
A5955_RS15950	3251632..3252030	+	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
A5955_RS15955	3252040..3252492	+	453	WP_001059871.1	GNAT family N-acetyltransferase	-
A5955_RS15960	3252811..3253014	+	204	Protein_3008	RNA ligase RtcB family protein	-
A5955_RS15965	3253013..3253534	+	522	Protein_3009	peptide chain release factor H	-
A5955_RS15970	3253591..3255048	-	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
A5955_RS15975	3255309..3255767	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
-	3256363..3256443	+	81	NuclAT_10	-	-
-	3256363..3256443	+	81	NuclAT_10	-	-
-	3256363..3256443	+	81	NuclAT_10	-	-
-	3256363..3256443	+	81	NuclAT_10	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T62745 WP_001263495.1 NZ_CP015241:3251632-3252030 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T62745 NZ_CP015241:3251632-3252030 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT62745 WP_000554757.1 NZ_CP015241:3251336-3251629 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT62745 NZ_CP015241:3251336-3251629 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6