Detailed information of TA system TA062744

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 3245283..3245824
• Replicon: chromosome
• Accession: NZ_CP015241
• Organism: Escherichia coli strain 2013C-4465

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: S1FHQ9
• Locus tag: A5955_RS15910
• Protein ID: WP_000615979.1
• Coordinates: 3245283..3245561 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: C3TP07
• Locus tag: A5955_RS15915
• Protein ID: WP_000729705.1
• Coordinates: 3245564..3245824 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A5955_RS15895	3242966..3243544	+	579	WP_000284050.1	D-sedoheptulose 7-phosphate isomerase	-
A5955_RS15900	3243649..3244416	+	768	WP_001301698.1	class II glutamine amidotransferase	-
A5955_RS15905	3244387..3245127	-	741	WP_001225679.1	murein L,D-transpeptidase	-
A5955_RS15910	3245283..3245561	-	279	WP_000615979.1	type II toxin-antitoxin system mRNA interferase toxin YafQ	Toxin
A5955_RS15915	3245564..3245824	-	261	WP_000729705.1	type II toxin-antitoxin system antitoxin DinJ	Antitoxin
A5955_RS15920	3246010..3246783	+	774	WP_000543891.1	C40 family peptidase	-
A5955_RS31105	3246959..3247456	+	498	Protein_3001	REP-associated tyrosine transposase RayT	-
A5955_RS15930	3247689..3249401	-	1713	Protein_3002	flagellar biosynthesis protein FlhA	-
A5955_RS15935	3249373..3250158	+	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (1-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-85)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10830.62 Da
• Isoelectric Point: 10.0702

>T62744 WP_000615979.1 NZ_CP015241:c3245561-3245283 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Nucleotide

• Length: 279 bp

>T62744 NZ_CP015241:c3245561-3245283 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9377.74 Da
• Isoelectric Point: 5.0758

>AT62744 WP_000729705.1 NZ_CP015241:c3245824-3245564 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGV

Nucleotide

• Length: 261 bp

>AT62744 NZ_CP015241:c3245824-3245564 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAGTTTAA

Structures

Toxin

• AlphaFold DB: S1FHQ9