Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 89206..89708 Replicon chromosome
Accession NZ_CP015241
Organism Escherichia coli strain 2013C-4465

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag A5955_RS00545 Protein ID WP_000767829.1
Coordinates 89206..89460 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag A5955_RS00550 Protein ID WP_001259255.1
Coordinates 89457..89708 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A5955_RS00520 84221..84448 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
A5955_RS00525 84462..85520 - 1059 WP_000492328.1 transport protein YeeE -
A5955_RS00530 85699..87057 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
A5955_RS30780 87047..87109 - 63 WP_010723108.1 membrane protein YoeI -
A5955_RS00535 87324..88253 - 930 WP_000803334.1 LysR family transcriptional regulator -
A5955_RS00540 88299..89123 - 825 WP_000754737.1 SDR family oxidoreductase -
A5955_RS00545 89206..89460 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
A5955_RS00550 89457..89708 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
A5955_RS27695 89992..90042 + 51 WP_013009236.1 his operon leader peptide -
A5955_RS00555 90188..91087 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
A5955_RS00560 91093..92397 + 1305 WP_001419115.1 histidinol dehydrogenase -
A5955_RS00565 92394..93464 + 1071 WP_000108999.1 histidinol-phosphate transaminase -
A5955_RS00570 93464..94531 + 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T62731 WP_000767829.1 NZ_CP015241:c89460-89206 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T62731 NZ_CP015241:c89460-89206 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT62731 WP_001259255.1 NZ_CP015241:c89708-89457 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT62731 NZ_CP015241:c89708-89457 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGCCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCTCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References