Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3032379..3032920 Replicon chromosome
Accession NZ_CP015240
Organism Escherichia coli strain 2011C-3911

Toxin (Protein)


Gene name yafQ Uniprot ID E9TC49
Locus tag A5956_RS15460 Protein ID WP_000615982.1
Coordinates 3032379..3032657 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag A5956_RS15465 Protein ID WP_000729704.1
Coordinates 3032660..3032920 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A5956_RS15445 3029961..3030539 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
A5956_RS15450 3030745..3031512 + 768 WP_000333380.1 class II glutamine amidotransferase -
A5956_RS15455 3031483..3032223 - 741 WP_001225679.1 murein L,D-transpeptidase -
A5956_RS15460 3032379..3032657 - 279 WP_000615982.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
A5956_RS15465 3032660..3032920 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
A5956_RS15470 3033106..3033879 + 774 WP_000543895.1 C40 family peptidase -
A5956_RS15475 3033936..3034292 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
A5956_RS15480 3034285..3034563 - 279 WP_000598758.1 type II toxin-antitoxin system HicA family toxin -
A5956_RS15485 3034668..3036380 - 1713 Protein_2792 flagellar biosynthesis protein FlhA -
A5956_RS15490 3036352..3037137 + 786 WP_000207557.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - rhs/PAAR / rhs/PAAR / gmhA/lpcA 3016965..3034563 17598


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10816.59 Da        Isoelectric Point: 10.0702

>T62718 WP_000615982.1 NZ_CP015240:c3032657-3032379 [Escherichia coli]
MIQRDIEYSGQFSKDVKVAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T62718 NZ_CP015240:c3032657-3032379 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAAGTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT62718 WP_000729704.1 NZ_CP015240:c3032920-3032660 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT62718 NZ_CP015240:c3032920-3032660 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References