Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 103487..103989 Replicon chromosome
Accession NZ_CP015240
Organism Escherichia coli strain 2011C-3911

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag A5956_RS01260 Protein ID WP_000767829.1
Coordinates 103487..103741 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag A5956_RS01265 Protein ID WP_001259255.1
Coordinates 103738..103989 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A5956_RS01235 98502..98729 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
A5956_RS01240 98743..99801 - 1059 WP_000492346.1 transport protein YeeE -
A5956_RS01245 99980..101338 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
A5956_RS27105 101328..101390 - 63 WP_010723108.1 membrane protein YoeI -
A5956_RS01250 101605..102534 - 930 WP_000803351.1 LysR family transcriptional regulator -
A5956_RS01255 102580..103404 - 825 WP_000754758.1 SDR family oxidoreductase -
A5956_RS01260 103487..103741 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
A5956_RS01265 103738..103989 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
A5956_RS24945 104273..104323 + 51 WP_001364200.1 his operon leader peptide -
A5956_RS01270 104469..105368 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
A5956_RS01275 105374..106678 + 1305 WP_001317939.1 histidinol dehydrogenase -
A5956_RS01280 106675..107745 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
A5956_RS01285 107745..108812 + 1068 WP_000080102.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T62710 WP_000767829.1 NZ_CP015240:c103741-103487 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T62710 NZ_CP015240:c103741-103487 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT62710 WP_001259255.1 NZ_CP015240:c103989-103738 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT62710 NZ_CP015240:c103989-103738 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATATAACTCCCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References