Detailed information of TA system TA062705

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 96229..96653
• Replicon: plasmid unnamed1
• Accession: NZ_CP015239
• Organism: Escherichia coli strain 2011C-3911

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: A5956_RS00485
• Protein ID: WP_032327044.1
• Coordinates: 96229..96390 (-)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	96455..96653 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A5956_RS00455	92014..92397	-	384	WP_001151535.1	relaxosome protein TraM	-
A5956_RS00460	92673..93320	+	648	WP_000602507.1	transglycosylase SLT domain-containing protein	-
A5956_RS00465	93616..94437	-	822	WP_001234499.1	DUF945 domain-containing protein	-
A5956_RS00470	94556..94843	-	288	WP_000107543.1	hypothetical protein	-
A5956_RS00475	95000..95302	-	303	WP_001272236.1	hypothetical protein	-
A5956_RS00485	96229..96390	-	162	WP_032327044.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	96455..96653	-	199	NuclAT_0	-	Antitoxin
-	96455..96653	-	199	NuclAT_0	-	Antitoxin
-	96455..96653	-	199	NuclAT_0	-	Antitoxin
-	96455..96653	-	199	NuclAT_0	-	Antitoxin
A5956_RS00495	96665..97384	-	720	WP_032327045.1	plasmid SOS inhibition protein A	-
A5956_RS00500	97381..97815	-	435	WP_032327046.1	conjugation system SOS inhibitor PsiB	-
A5956_RS00505	97870..99834	-	1965	WP_064055974.1	ParB/RepB/Spo0J family partition protein	-
A5956_RS00510	99900..100133	-	234	WP_000005990.1	DUF905 family protein	-
A5956_RS00515	100190..100729	-	540	WP_032326923.1	single-stranded DNA-binding protein	-
A5956_RS00520	100755..100961	-	207	WP_000547940.1	hypothetical protein	-
A5956_RS27615	101031..101558	+	528	WP_064055975.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..125977	125977

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 6149.36 Da
• Isoelectric Point: 9.1977

>T62705 WP_032327044.1 NZ_CP015239:c96390-96229 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVTATMAYESGGK

Nucleotide

• Length: 162 bp

>T62705 NZ_CP015239:c96390-96229 [Escherichia coli]
ATGAAACTACCACGCAGCTCTCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGATACAGGGAAGTTACGGCAACAATGGCTTACGAATCCGGCGGTAAGT
AG

Antitoxin

Nucleotide

• Length: 199 bp

>AT62705 NZ_CP015239:c96653-96455 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin