Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2691527..2692029 Replicon chromosome
Accession NZ_CP015229
Organism Escherichia coli strain 06-00048

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag GJ11_RS15595 Protein ID WP_000767829.1
Coordinates 2691527..2691781 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag GJ11_RS15600 Protein ID WP_001259255.1
Coordinates 2691778..2692029 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJ11_RS15565 2686542..2686769 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
GJ11_RS15570 2686783..2687841 - 1059 WP_065227176.1 transport protein YeeE -
GJ11_RS15575 2688020..2689378 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
GJ11_RS15580 2689368..2689430 - 63 WP_010723108.1 membrane protein YoeI -
GJ11_RS15585 2689645..2690574 - 930 WP_065227177.1 LysR family transcriptional regulator -
GJ11_RS15590 2690620..2691444 - 825 WP_000754737.1 SDR family oxidoreductase -
GJ11_RS15595 2691527..2691781 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
GJ11_RS15600 2691778..2692029 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
GJ11_RS15605 2692313..2692363 + 51 WP_001364200.1 his operon leader peptide -
GJ11_RS15610 2692509..2693408 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
GJ11_RS15615 2693414..2694718 + 1305 WP_065227178.1 histidinol dehydrogenase -
GJ11_RS15620 2694715..2695797 + 1083 WP_065227179.1 histidinol-phosphate transaminase -
GJ11_RS15625 2695797..2696864 + 1068 WP_065227180.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T62693 WP_000767829.1 NZ_CP015229:c2691781-2691527 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T62693 NZ_CP015229:c2691781-2691527 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT62693 WP_001259255.1 NZ_CP015229:c2692029-2691778 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT62693 NZ_CP015229:c2692029-2691778 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCCCTGGAGGAGACGGCTTATC
TACTGCGTTCCCCCGCTAATGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCTGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References