Detailed information of TA system TA062678

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 1663280..1663651
• Replicon: chromosome
• Accession: NZ_CP015229
• Organism: Escherichia coli strain 06-00048

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: D6I8D3
• Locus tag: GJ11_RS09980
• Protein ID: WP_001307771.1
• Coordinates: 1663457..1663651 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	1663280..1663458 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJ11_RS09955	1659237..1660610	+	1374	WP_065226721.1	ATP-dependent RNA helicase DbpA	-
GJ11_RS09960	1660737..1661672	-	936	WP_001157407.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
GJ11_RS09965	1661724..1662959	-	1236	WP_000040852.1	site-specific integrase	-
GJ11_RS09970	1662961..1663176	-	216	WP_000079604.1	excisionase XisR	-
GJ11_RS09975	1663276..1663464	-	189	WP_001307770.1	DUF1187 family protein	-
-	1663280..1663458	+	179	NuclAT_0	-	Antitoxin
-	1663280..1663458	+	179	NuclAT_0	-	Antitoxin
-	1663280..1663458	+	179	NuclAT_0	-	Antitoxin
-	1663280..1663458	+	179	NuclAT_0	-	Antitoxin
GJ11_RS09980	1663457..1663651	-	195	WP_001307771.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
GJ11_RS09985	1663684..1664364	-	681	WP_021572575.1	YqaJ viral recombinase family protein	-
GJ11_RS09990	1664361..1665140	-	780	WP_047671032.1	phage recombination protein Bet	-
GJ11_RS09995	1665146..1665442	-	297	WP_000102059.1	host-nuclease inhibitor protein Gam	-
GJ11_RS10000	1665420..1667443	-	2024	Protein_1572	hypothetical protein	-
GJ11_RS10005	1667542..1667816	-	275	Protein_1573	hypothetical protein	-
GJ11_RS10010	1667891..1668061	-	171	WP_077816938.1	hypothetical protein	-
GJ11_RS10015	1668061..1668282	-	222	WP_000560223.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1661724..1707380	45656

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 6986.91 Da
• Isoelectric Point: 9.6161

>T62678 WP_001307771.1 NZ_CP015229:c1663651-1663457 [Escherichia coli]
VRYTKVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T62678 NZ_CP015229:c1663651-1663457 [Escherichia coli]
GTGCGATACACAAAAGTCAAACCATGCCCTTTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
CGCGAAGTGTAACGGATGCGAATCCCGAACCGGCTATGGTGGAAGTGAAAAAGAAGCACTCGAACGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT62678 NZ_CP015229:1663280-1663458 [Escherichia coli]
GAGGACAGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTCCCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAATTTAGATTTTGAATATCTTATCCAGTTAGTAGGAGTGCCACCTTCTTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7H9KGA4

Antitoxin