Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 325547..326241 Replicon chromosome
Accession NZ_CP015229
Organism Escherichia coli strain 06-00048

Toxin (Protein)


Gene name yafO Uniprot ID A0A193LMV6
Locus tag GJ11_RS03485 Protein ID WP_065226184.1
Coordinates 325843..326241 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag GJ11_RS03480 Protein ID WP_000554755.1
Coordinates 325547..325840 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJ11_RS03455 321364..321831 + 468 WP_065226180.1 flagellar basal body-associated FliL family protein -
GJ11_RS03460 321851..322567 + 717 WP_065226181.1 FliA/WhiG family RNA polymerase sigma factor -
GJ11_RS03465 322580..323443 + 864 WP_001169518.1 flagellar motor stator protein MotA -
GJ11_RS03470 323446..324369 + 924 WP_065226182.1 putative lateral flagellar export/assembly protein LafU -
GJ11_RS03475 324440..325495 + 1056 WP_065226183.1 DNA polymerase IV -
GJ11_RS03480 325547..325840 + 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
GJ11_RS03485 325843..326241 + 399 WP_065226184.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
GJ11_RS03490 326251..326703 + 453 WP_071906053.1 GNAT family N-acetyltransferase -
GJ11_RS03495 327022..328161 + 1140 WP_065226186.1 RNA ligase RtcB family protein -
GJ11_RS03500 328158..328772 + 615 WP_065226187.1 peptide chain release factor H -
GJ11_RS03505 328829..330286 - 1458 WP_001293002.1 cytosol nonspecific dipeptidase -
GJ11_RS03510 330547..331005 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15447.82 Da        Isoelectric Point: 8.0949

>T62670 WP_065226184.1 NZ_CP015229:325843-326241 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPSQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T62670 NZ_CP015229:325843-326241 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGTCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT62670 WP_000554755.1 NZ_CP015229:325547-325840 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT62670 NZ_CP015229:325547-325840 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAACTGGTGAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A193LMV6

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References